Monitoring of the heat-shock response in Escherichia coli using an optical biosensor

A surface plasmon resonance (SPR) method for monitoring the concentration of the chaperone DnaK and its relation to physiological stress response in a recombinant Escherichia coli strain subjected to heat shock is described. The DnaK protein, an abundantly occurring representative of the heat-shock...

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Bibliographic Details
Published in:Analytical biochemistry 2003-11, Vol.322 (2), p.156-163
Main Authors: Vostiar, Igor, Tkac, Jan, Mandenius, Carl-Fredrik
Format: Article
Language:English
Subjects:
Biochemistry - methods
Biosensing Techniques
Buffers
DnaK analysis
DnaK protein
Enzyme-Linked Immunosorbent Assay - methods
Escherichia coli
Escherichia coli - physiology
Escherichia coli Proteins - analysis
Escherichia coli Proteins - immunology
Escherichia coli Proteins - metabolism
Heat shock response analysis
Heat-Shock Response - physiology
HSP70 Heat-Shock Proteins - analysis
HSP70 Heat-Shock Proteins - immunology
HSP70 Heat-Shock Proteins - metabolism
Optics and Photonics
Reproducibility of Results
Specimen Handling - methods
Stress response sensor
Surface plasmon resonance
Surface Plasmon Resonance - methods
TECHNOLOGY
TEKNIKVETENSKAP
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Summary:A surface plasmon resonance (SPR) method for monitoring the concentration of the chaperone DnaK and its relation to physiological stress response in a recombinant Escherichia coli strain subjected to heat shock is described. The DnaK protein, an abundantly occurring representative of the heat-shock proteins, was used as a marker of physiological stress. The SPR biosensor instrument was used for label-free immunoaffinity detection directly in cell culture lysates using an anti-DnaK monoclonal IgG antibody immobilized on the sensor surface. The SPR method provides a fast response (
ISSN:0003-2697
1096-0309
1096-0309
DOI:10.1016/j.ab.2003.07.019