A high-density poly(ethylene glycol) polymer brush for immobilization on glass-type surfaces

Label-free heterogeneous phase detection critically depends on the properties of the interfacial layer. We have obtained high-density monomolecular poly(ethylene glycol) (PEG) layers by solvent-free coupling of homo-bifunctional PEGs (2000 g/mol) at 75°C to silica surfaces silanized with glycidyloxi...

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Bibliographic Details
Published in:Biosensors & bioelectronics 2000-11, Vol.15 (9), p.473-481
Main Authors: Piehler, Jacob, Brecht, Andreas, Valiokas, Ramūnas, Liedberg, Bo, Gauglitz, Günter
Format: Article
Language:English
Subjects:
Affinity interaction
Animals
Biological and medical sciences
Biosensing Techniques - methods
Biosensors
Biotechnology
Contact angle
Drug Stability
Ellipsometry
Enzyme immobilization
Fundamental and applied biological sciences. Psychology
Glass
Heterogeneous phase detection
High density polyethylenes
Immobilization
In Vitro Techniques
Interfaces (materials)
Ligands
Methods. Procedures. Technologies
Ovalbumin
Poly(ethylene glycol)
Polyethylene Glycols
Protein Binding
Reflectometric interference spectroscopy
Silicon Dioxide
Spectroscopy
Surface Properties
TECHNOLOGY
TEKNIKVETENSKAP
Various methods and equipments
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Summary:Label-free heterogeneous phase detection critically depends on the properties of the interfacial layer. We have obtained high-density monomolecular poly(ethylene glycol) (PEG) layers by solvent-free coupling of homo-bifunctional PEGs (2000 g/mol) at 75°C to silica surfaces silanized with glycidyloxipropyltrimethoxysilane (GOPTS). Characterization by ellipsometry and contact angles revealed that PEG layers up to 3.4 ng/mm 2 with low roughness and flexibility were obtained. Specific and non-specific binding at these PEG surfaces was monitored by reflectometric interference spectroscopy (RIfS). No significant non-specific adsorption upon incubation of 1 mg/ml ovalbumin was detectable (
ISSN:0956-5663
1873-4235
1873-4235
DOI:10.1016/S0956-5663(00)00104-4