Expression and significance of FXYD-3 protein in gastric adenocarcinoma

• Published in: Disease markers 2010-01, Vol.28 (2), p.63-69
• Main Authors: Zhu, Zhen-Long, Zhao, Zeng-Ren, Zhang, Yu, Yang, Yan-Hong, Wang, Zheng-Min, Cui, Dong-Sheng, Wang, Ming-Wei, Kleeff, Jörg, Kayed, Hany, Yan, Bao-Yong, Sun, Xiao-Feng
• Format: Article
• Language: English
• Subjects: FXYD-3; gastric cancer; immunohistochemistry; MEDICIN; MEDICINE; Other
• ISSN: 0278-0240; 1875-8630
• DOI: 10.3233/DMA-2010-0669

OBJECTIVEFXYD-3, also known as Mat-8, is a member of the FXYD protein family. It was reported that this protein can associate with and modify the transport properties of Na, K-ATPase, and may play an important role in a variety of physiological and pathological states. This protein is up-regulated in certain types of cancers (such as breast, prostate and pancreatic cancer), but down-regulated in other types of cancers (such as colon and kidney cancer). No study has been performed in gastric cancer; therefore, the aim of this project was to investigate FXYD-3 expression and its clinicopathological significance in gastric adenocarcinoma. PATIENTS AND METHODSFXYD-3 protein was examined by immunohistochemistry in normal gastric mucous (n= 29) and gastric adenocarcinoma (n=51), obtained from surgical resection of gastric cancer patients. RESULTSFXYD-3 protein was present in the cytoplasm of normal gastric epithelial cells or gastric cancer cells. The rate of FXYD-3 strong expression was significantly higher in cancer (51% of 51) than in normal mucosa (10% of 29, X;{2}=13.210, p < 0.0001). FXYD-3 expressed strongly in ulcerative/infiltrating types of cancers compared to polypoid/fungating ones (X;{2}=5.765, p=0.016). However, FXYD-3 expression was not correlated with patient's gender, age, tumor size, lymph node status and histological grade (p > 0.05). Conclosion: Up-regulated expression of FXYD-3 protein may be involved in tumourgenesis and invasion of gastric adenocarcinoma.