Carbon and gold electrodes as electrochemical transducers for DNA hybridisation sensors

Genosensor technology relying on the use of carbon and gold electrodes is reviewed. The key steps of each analytical procedure, namely DNA-probe immobilisation, hybridisation, labelling and electrochemical investigation of the surface, are discussed in detail with separate sections devoted to label-...

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Bibliographic Details
Published in:Biosensors & bioelectronics 2004-01, Vol.19 (6), p.515-530
Main Authors: Lucarelli, Fausto, Marrazza, Giovanna, Turner, Anthony P.F, Mascini, Marco
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Biosensing Techniques - instrumentation
Biosensing Techniques - methods
Biosensors
Biotechnology
Carbon
Carbon electrodes
Coated Materials, Biocompatible - chemical synthesis
DNA - analysis
DNA - chemistry
DNA - genetics
DNA chips
DNA hybridisation
DNA Probes - chemical synthesis
DNA sensors
Electrochemical DNA biosensors
Electrochemistry - instrumentation
Electrochemistry - methods
Electrodes
Equipment Design
Fundamental and applied biological sciences. Psychology
Genosensors
Gold
Gold electrodes
Humans
In Situ Hybridization - instrumentation
In Situ Hybridization - methods
Methods. Procedures. Technologies
PCR
Polymerase Chain Reaction - instrumentation
Polymerase Chain Reaction - methods
TECHNOLOGY
TEKNIKVETENSKAP
Transducers
Various methods and equipments
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Description
Summary:Genosensor technology relying on the use of carbon and gold electrodes is reviewed. The key steps of each analytical procedure, namely DNA-probe immobilisation, hybridisation, labelling and electrochemical investigation of the surface, are discussed in detail with separate sections devoted to label-free and newly emerging magnetic assays. Special emphasis has been given to protocols that have been used with real DNA samples.
ISSN:0956-5663
1873-4235
1873-4235
DOI:10.1016/S0956-5663(03)00256-2