Direct monitoring of formaldehyde vapour and detection of ethanol vapour using dehydrogenase-based biosensors

Biosensors capable of directly detecting low levels of formaldehyde and ethanol vapour were constructed, Both biosensors are based on dehydrogenase enzymes which produce reduced nicotinamide adenine dinucleotide as part of the oxidation of formaldehyde and ethanol, The enzymes were immobilized in a...

Full description

Saved in:
Bibliographic Details
Published in:Analyst (London) 1996, Vol.121 (12), p.1769-1773
Main Authors: DENNISON, M. J, HALL, J. M, TURNER, A. P. F
Format: Article
Language:English
Subjects:
Analytical chemistry
Biological and medical sciences
Biosensors
Biotechnology
Chemistry
Exact sciences and technology
Fundamental and applied biological sciences. Psychology
General, instrumentation
Methods. Procedures. Technologies
TECHNOLOGY
TEKNIKVETENSKAP
Various methods and equipments
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
cited_by cdi_FETCH-LOGICAL-c323t-f3df8c7269c41f58da18a5e6eff4c4924a7b6ba3f5105d3d4b32626ad0923c6c3
cites
container_end_page 1773
container_issue 12
container_start_page 1769
container_title Analyst (London)
container_volume 121
creator DENNISON, M. J
HALL, J. M
TURNER, A. P. F
description Biosensors capable of directly detecting low levels of formaldehyde and ethanol vapour were constructed, Both biosensors are based on dehydrogenase enzymes which produce reduced nicotinamide adenine dinucleotide as part of the oxidation of formaldehyde and ethanol, The enzymes were immobilized in a reverse micelle medium which did not dehydrate significantly over time, and allowed direct gas-phase monitoring, A screen-printed electrode was used as transducer. Formaldehyde and ethanol vapour partitioned into the reverse micelle media, where it was acted upon by the relevant enzyme, Reduced nicotinamide adenine dinucleotide was oxidized at the working electrode at a potential of 800 mV versus an Ag/AgCl reference electrode, Formaldehyde could be measured over the concentration range 1 ppb-1.3 ppm and ethanol could be detected over the range 50-250 ppm.
doi_str_mv 10.1039/AN9962101769
format article
fullrecord <record><control><sourceid>swepub_cross</sourceid><recordid>TN_cdi_swepub_primary_oai_DiVA_org_liu_65264</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>oai_DiVA_org_liu_65264</sourcerecordid><originalsourceid>FETCH-LOGICAL-c323t-f3df8c7269c41f58da18a5e6eff4c4924a7b6ba3f5105d3d4b32626ad0923c6c3</originalsourceid><addsrcrecordid>eNpFkEtPwzAMgCMEEmNw4wf0wJFCXs3a47Txkia4ANfIzWML6pIqaUH797QMxsWW5e-zbCN0SfANway6nT9XlaAEk5mojtCEMMHzoqDlMZpgjFlORcFP0VlKH0NJcIEnaLt00agu2wbvuhCdX2fBZjbELTTabHbaZJ_Qhj5m4HWmTTfALvgRMt0GfGj--n0a5R8nhrXxkExeD0FntQvJ-BRiOkcnFppkLn7zFL3d370uHvPVy8PTYr7KFaOsyy3TtlQzKirFiS1KDaSEwghjLVe8ohxmtaiB2WI4QjPNa0YFFaBxRZkSik3R9X5u-jJtX8s2ui3EnQzg5NK9z2WIa9m4XoqCCv6PqxhSisYeBILl-FkJ_v-zA361x1tIChobwSuXDg4dt6KEfQN2O3tL</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>Direct monitoring of formaldehyde vapour and detection of ethanol vapour using dehydrogenase-based biosensors</title><source>Royal Society of Chemistry: Jisc Collections: Journals Archive 1841-2007 (2019-2023)</source><creator>DENNISON, M. J ; HALL, J. M ; TURNER, A. P. F</creator><creatorcontrib>DENNISON, M. J ; HALL, J. M ; TURNER, A. P. F</creatorcontrib><description>Biosensors capable of directly detecting low levels of formaldehyde and ethanol vapour were constructed, Both biosensors are based on dehydrogenase enzymes which produce reduced nicotinamide adenine dinucleotide as part of the oxidation of formaldehyde and ethanol, The enzymes were immobilized in a reverse micelle medium which did not dehydrate significantly over time, and allowed direct gas-phase monitoring, A screen-printed electrode was used as transducer. Formaldehyde and ethanol vapour partitioned into the reverse micelle media, where it was acted upon by the relevant enzyme, Reduced nicotinamide adenine dinucleotide was oxidized at the working electrode at a potential of 800 mV versus an Ag/AgCl reference electrode, Formaldehyde could be measured over the concentration range 1 ppb-1.3 ppm and ethanol could be detected over the range 50-250 ppm.</description><identifier>ISSN: 0003-2654</identifier><identifier>ISSN: 1364-5528</identifier><identifier>EISSN: 1364-5528</identifier><identifier>DOI: 10.1039/AN9962101769</identifier><identifier>CODEN: ANALAO</identifier><language>eng</language><publisher>Cambridge: Royal Society of Chemistry</publisher><subject>Analytical chemistry ; Biological and medical sciences ; Biosensors ; Biotechnology ; Chemistry ; Exact sciences and technology ; Fundamental and applied biological sciences. Psychology ; General, instrumentation ; Methods. Procedures. Technologies ; TECHNOLOGY ; TEKNIKVETENSKAP ; Various methods and equipments</subject><ispartof>Analyst (London), 1996, Vol.121 (12), p.1769-1773</ispartof><rights>1997 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c323t-f3df8c7269c41f58da18a5e6eff4c4924a7b6ba3f5105d3d4b32626ad0923c6c3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,309,310,314,780,784,789,790,885,4024,4050,4051,23930,23931,25140,27923,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&amp;idt=2510521$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-65264$$DView record from Swedish Publication Index$$Hfree_for_read</backlink></links><search><creatorcontrib>DENNISON, M. J</creatorcontrib><creatorcontrib>HALL, J. M</creatorcontrib><creatorcontrib>TURNER, A. P. F</creatorcontrib><title>Direct monitoring of formaldehyde vapour and detection of ethanol vapour using dehydrogenase-based biosensors</title><title>Analyst (London)</title><description>Biosensors capable of directly detecting low levels of formaldehyde and ethanol vapour were constructed, Both biosensors are based on dehydrogenase enzymes which produce reduced nicotinamide adenine dinucleotide as part of the oxidation of formaldehyde and ethanol, The enzymes were immobilized in a reverse micelle medium which did not dehydrate significantly over time, and allowed direct gas-phase monitoring, A screen-printed electrode was used as transducer. Formaldehyde and ethanol vapour partitioned into the reverse micelle media, where it was acted upon by the relevant enzyme, Reduced nicotinamide adenine dinucleotide was oxidized at the working electrode at a potential of 800 mV versus an Ag/AgCl reference electrode, Formaldehyde could be measured over the concentration range 1 ppb-1.3 ppm and ethanol could be detected over the range 50-250 ppm.</description><subject>Analytical chemistry</subject><subject>Biological and medical sciences</subject><subject>Biosensors</subject><subject>Biotechnology</subject><subject>Chemistry</subject><subject>Exact sciences and technology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>General, instrumentation</subject><subject>Methods. Procedures. Technologies</subject><subject>TECHNOLOGY</subject><subject>TEKNIKVETENSKAP</subject><subject>Various methods and equipments</subject><issn>0003-2654</issn><issn>1364-5528</issn><issn>1364-5528</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1996</creationdate><recordtype>article</recordtype><recordid>eNpFkEtPwzAMgCMEEmNw4wf0wJFCXs3a47Txkia4ANfIzWML6pIqaUH797QMxsWW5e-zbCN0SfANway6nT9XlaAEk5mojtCEMMHzoqDlMZpgjFlORcFP0VlKH0NJcIEnaLt00agu2wbvuhCdX2fBZjbELTTabHbaZJ_Qhj5m4HWmTTfALvgRMt0GfGj--n0a5R8nhrXxkExeD0FntQvJ-BRiOkcnFppkLn7zFL3d370uHvPVy8PTYr7KFaOsyy3TtlQzKirFiS1KDaSEwghjLVe8ohxmtaiB2WI4QjPNa0YFFaBxRZkSik3R9X5u-jJtX8s2ui3EnQzg5NK9z2WIa9m4XoqCCv6PqxhSisYeBILl-FkJ_v-zA361x1tIChobwSuXDg4dt6KEfQN2O3tL</recordid><startdate>1996</startdate><enddate>1996</enddate><creator>DENNISON, M. J</creator><creator>HALL, J. M</creator><creator>TURNER, A. P. F</creator><general>Royal Society of Chemistry</general><scope>IQODW</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>ADTPV</scope><scope>AOWAS</scope><scope>DG8</scope></search><sort><creationdate>1996</creationdate><title>Direct monitoring of formaldehyde vapour and detection of ethanol vapour using dehydrogenase-based biosensors</title><author>DENNISON, M. J ; HALL, J. M ; TURNER, A. P. F</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c323t-f3df8c7269c41f58da18a5e6eff4c4924a7b6ba3f5105d3d4b32626ad0923c6c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1996</creationdate><topic>Analytical chemistry</topic><topic>Biological and medical sciences</topic><topic>Biosensors</topic><topic>Biotechnology</topic><topic>Chemistry</topic><topic>Exact sciences and technology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>General, instrumentation</topic><topic>Methods. Procedures. Technologies</topic><topic>TECHNOLOGY</topic><topic>TEKNIKVETENSKAP</topic><topic>Various methods and equipments</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>DENNISON, M. J</creatorcontrib><creatorcontrib>HALL, J. M</creatorcontrib><creatorcontrib>TURNER, A. P. F</creatorcontrib><collection>Pascal-Francis</collection><collection>CrossRef</collection><collection>SwePub</collection><collection>SwePub Articles</collection><collection>SWEPUB Linköpings universitet</collection><jtitle>Analyst (London)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>DENNISON, M. J</au><au>HALL, J. M</au><au>TURNER, A. P. F</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Direct monitoring of formaldehyde vapour and detection of ethanol vapour using dehydrogenase-based biosensors</atitle><jtitle>Analyst (London)</jtitle><date>1996</date><risdate>1996</risdate><volume>121</volume><issue>12</issue><spage>1769</spage><epage>1773</epage><pages>1769-1773</pages><issn>0003-2654</issn><issn>1364-5528</issn><eissn>1364-5528</eissn><coden>ANALAO</coden><abstract>Biosensors capable of directly detecting low levels of formaldehyde and ethanol vapour were constructed, Both biosensors are based on dehydrogenase enzymes which produce reduced nicotinamide adenine dinucleotide as part of the oxidation of formaldehyde and ethanol, The enzymes were immobilized in a reverse micelle medium which did not dehydrate significantly over time, and allowed direct gas-phase monitoring, A screen-printed electrode was used as transducer. Formaldehyde and ethanol vapour partitioned into the reverse micelle media, where it was acted upon by the relevant enzyme, Reduced nicotinamide adenine dinucleotide was oxidized at the working electrode at a potential of 800 mV versus an Ag/AgCl reference electrode, Formaldehyde could be measured over the concentration range 1 ppb-1.3 ppm and ethanol could be detected over the range 50-250 ppm.</abstract><cop>Cambridge</cop><pub>Royal Society of Chemistry</pub><doi>10.1039/AN9962101769</doi><tpages>5</tpages></addata></record>
fulltext fulltext
identifier ISSN: 0003-2654
ispartof Analyst (London), 1996, Vol.121 (12), p.1769-1773
issn 0003-2654
1364-5528
1364-5528
language eng
recordid cdi_swepub_primary_oai_DiVA_org_liu_65264
source Royal Society of Chemistry: Jisc Collections: Journals Archive 1841-2007 (2019-2023)
subjects Analytical chemistry
Biological and medical sciences
Biosensors
Biotechnology
Chemistry
Exact sciences and technology
Fundamental and applied biological sciences. Psychology
General, instrumentation
Methods. Procedures. Technologies
TECHNOLOGY
TEKNIKVETENSKAP
Various methods and equipments
title Direct monitoring of formaldehyde vapour and detection of ethanol vapour using dehydrogenase-based biosensors
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-01T20%3A38%3A51IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-swepub_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Direct%20monitoring%20of%20formaldehyde%20vapour%20and%20detection%20of%20ethanol%20vapour%20using%20dehydrogenase-based%20biosensors&rft.jtitle=Analyst%20(London)&rft.au=DENNISON,%20M.%20J&rft.date=1996&rft.volume=121&rft.issue=12&rft.spage=1769&rft.epage=1773&rft.pages=1769-1773&rft.issn=0003-2654&rft.eissn=1364-5528&rft.coden=ANALAO&rft_id=info:doi/10.1039/AN9962101769&rft_dat=%3Cswepub_cross%3Eoai_DiVA_org_liu_65264%3C/swepub_cross%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c323t-f3df8c7269c41f58da18a5e6eff4c4924a7b6ba3f5105d3d4b32626ad0923c6c3%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_id=info:pmid/&rfr_iscdi=true