Hierachically Structured Hollow Silica Spheres for High Efficiency Immobilization of Enzymes

In this work, the first example of a hierarchically structured hollow silica system is reported without any chemical modification to the enzyme involved in the process. The leaching of the physically adsorbed enzyme is substantially restrained in comparison to pure hollow silica supports. The hierar...

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Bibliographic Details
Published in:Advanced functional materials 2013-05, Vol.23 (17), p.2162-2167
Main Authors: Cao, Shunsheng, Fang, Long, Zhao, Zhiyuan, Ge, Yi, Piletsky, Sergey, Turner, Anthony P. F.
Format: Article
Language:English
Subjects:
Architecture
Biochemistry
enzyme immobilization
Enzymes
Glucose oxidase
hierarchically structured particles
hollow silica
Immobilization
Leaching
Silicon dioxide
Stability
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Summary:In this work, the first example of a hierarchically structured hollow silica system is reported without any chemical modification to the enzyme involved in the process. The leaching of the physically adsorbed enzyme is substantially restrained in comparison to pure hollow silica supports. The hierarchical architecture is composed of the ordered hollow silica spheres with a shell‐in‐shell structure. This rationally integrated architecture, which serves as the host for glucose oxidase immobilization, displays many significant advantages, including increases in mechanical stability, enzyme loading, and bioactivity, and a decrease in enzyme leaching compared to existing pure hollow silica matrices. This facilitates further multifarious applications for enhanced enzyme immobilization, biosensors, and biocatalysis. The enhanced surface area and higher stability of the hierarchically structured hollow silica are shown to offer significant advantages for the encapsulation of glucose oxidase from aqueous media. Enhanced loading, enhanced bioactivity, and decreased leaching are demonstrated.
ISSN:1616-301X
1616-3028
1616-3028
DOI:10.1002/adfm.201201793