Recovery of Clostridium thermosulfurogenes produced beta-amylase by (hydroxypropyl)methylcellulose partition

A procedure for recovering Clostridium thermosulfurogenes produced β‐amylase from fermentation broth by partition was developed. The partition was achieved by addition of ammonium sulfate to an aqueous solution of the enzyme with (hydroxypropyl)methylcellulose. The β‐amylase‐containing pellet formed...

Full description

Saved in:
Bibliographic Details
Published in:Biotechnology progress 1990-05, Vol.6 (3), p.214-219
Main Authors: Miranda, E.A. (Michigan State University, East Lansing, MI), Berglund, K.A
Format: Article
Language:English
Subjects:
BETA AMILASA
BETA AMYLASE
beta-Amylase - isolation & purification
Biochemical Process Engineering
Biokemisk processteknik
Biotechnology
CELLULOSE
CELLULOSES
CELULOSA
Chemistry Techniques, Analytical - methods
CLOSTRIDIUM
Clostridium - enzymology
COLLOIDE
COLLOIDS
COLOIDES
DERIVATIVES
Ethers
Fermentation
FERMENTED PRODUCTS
Gels
hydroxypropyl methylcellulose
Hypromellose Derivatives
Methylcellulose - analogs & derivatives
PRODUCTOS FERMENTADOS
PRODUIT FERMENTE
SEPARACION
SEPARATING
SEPARATION
Temperature
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:A procedure for recovering Clostridium thermosulfurogenes produced β‐amylase from fermentation broth by partition was developed. The partition was achieved by addition of ammonium sulfate to an aqueous solution of the enzyme with (hydroxypropyl)methylcellulose. The β‐amylase‐containing pellet formed upon centrifugation could be redissolved and the polymer recovered by a second salt addition. The process was not dependent on polymer/enzyme solution pH, but it was affected by temperature, polymer nominal molecular weight and loading, and fermentation carbon source. Unlike more traditional aqueous‐phase partitions, such as poly(ethylene glycol)/dextran, the current approach appeared to be biospecific.
ISSN:8756-7938
1520-6033
1520-6033
DOI:10.1021/bp00003a010