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Characterisation of the volatile profile of orange juice contaminated with Alicyclobacillus acidoterrestris

A rapid and reliable analytical method, based on the characterisation of the volatile profile by dynamic headspace extraction followed by gas chromatography mass–spectrometry, was developed in order to early detect Alicyclobacillus acidoterrestris spoilage in orange juice. Gas chromatographic peak a...

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Published in:Food chemistry 2010-12, Vol.123 (3), p.653-658
Main Authors: Bianchi, F., Careri, M., Mangia, A., Mattarozzi, M., Musci, M., Concina, I., Gobbi, E.
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container_title Food chemistry
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creator Bianchi, F.
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description A rapid and reliable analytical method, based on the characterisation of the volatile profile by dynamic headspace extraction followed by gas chromatography mass–spectrometry, was developed in order to early detect Alicyclobacillus acidoterrestris spoilage in orange juice. Gas chromatographic peak areas were submitted to multivariate statistical analysis (principal component and linear discriminant analysis) in order to visualise clusters within samples and to detect the volatile compounds able to differentiate contaminated from not-contaminated samples. Significant differences in the volatile profile of the analysed samples were found, assessing the reliability of the proposed method to detect the A. acidoterrestris contamination in orange juice. Neither guaiacol nor 2,6-dibromophenol, usually regarded as A. acidoterrestris contamination markers, were detected in the analysed samples.
doi_str_mv 10.1016/j.foodchem.2010.05.023
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subjects 2,6-Dibromophenol
Alicyclobacillus acidoterrestris
analytical methods
bacterial contamination
Biological and medical sciences
detection
food analysis
food composition
Food contamination
Food industries
food spoilage
Fruit and vegetable industries
Fundamental and applied biological sciences. Psychology
gas chromatography
Guaiacol
headspace analysis
mass spectrometry
Multivariate analysis
Orange juice
rapid methods
reliability
volatile compounds
Volatile profile
title Characterisation of the volatile profile of orange juice contaminated with Alicyclobacillus acidoterrestris
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