Epitope approach in molecular imprinting of antibodies

Herein an approach to prepare molecularly imprinted polymer nanoparticles (nanoMIPs) with specific binding affinity for antibodies is reported. The process relied on the covalent immobilization of the template (whole immunoglobulin G (IgG), Fc domain of human IgG and peptide epitope) onto the surfac...

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Bibliographic Details
Published in:Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Analytical technologies in the biomedical and life sciences, 2019-08, Vol.1124, p.1-6
Main Authors: Moczko, Ewa, Guerreiro, Antonio, Cáceres, César, Piletska, Elena, Sellergren, Börje, Piletsky, Sergey A.
Format: Article
Language:English
Subjects:
Affinity chromatography
Animals
Antibodies
Epitopes - chemistry
Goats
Humans
Immunoglobulin G - chemistry
Molecular Imprinting
Molecular imprinting polymers
Nanoparticles
Nanoparticles - chemistry
Polymerization
Polymers - chemical synthesis
Polymers - chemistry
Protein Domains
Protein epitope
Solid-phase synthesis
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Summary:Herein an approach to prepare molecularly imprinted polymer nanoparticles (nanoMIPs) with specific binding affinity for antibodies is reported. The process relied on the covalent immobilization of the template (whole immunoglobulin G (IgG), Fc domain of human IgG and peptide epitope) onto the surface of a solid support, polymerization and affinity separation of nanoMIPs. The binding between nanoMIPs and their corresponding templates was analyzed and evaluated as being in sub-nanomolar and nano-molar range. The nanoMIPs prepared for Fc domain and epitope demonstrated a specific recognition of both human and goat IgGs, therefore they could be considered as a synthetic analogue of protein A and benefit from its intrinsic stability, short time and low cost of preparation. •The synthesis of nanoMIPs with specific binding affinity for antibodies•The nanoMIPs were prepared by the solid-phase approach and imprinted against whole IgG, Fc domain of human IgG and the peptide epitope.•Polymeric nanoparticles were able to bind IgG molecules with sub-nanomolar and nano-molar affinity.•New receptors can be considered as synthetic analogues of protein A for affinity chromatography, particularly monoclonal antibodies and antibodies fragments separation.•NanoMIPs benefit from its intrinsic stability, short time and low cost of preparation.
ISSN:1570-0232
1873-376X
1873-376X
DOI:10.1016/j.jchromb.2019.05.024