Analysis of hydroxamate siderophores in soil solution using liquid chromatography with mass spectrometry and tandem mass spectrometry with on-line sample preconcentration

A liquid chromatography with electrospray ionization mass spectrometry method was developed to quantitatively and qualitatively analyze 13 hydroxamate siderophores (ferrichrome, ferrirubin, ferrirhodin, ferrichrysin, ferricrocin, ferrioxamine B, D1, E and G, neocoprogen I and II, coprogen and triace...

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Bibliographic Details
Published in:Journal of separation science 2015-10, Vol.38 (19), p.3305-3312
Main Authors: Olofsson, Madelen A., Bylund, Dan
Format: Article
Language:English
Subjects:
Chromatography
Collision induced dissociation
Collision induces dissociation
Column-switching
Columns (structural)
Coprogens/Fusigens
Ferrichromes
Ferrioxamines
Ions
Liquid chromatography
Mass spectrometry
Methyl alcohol
Monitoring
On-line systems
Scientific imaging
Separation
Soil (material)
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Summary:A liquid chromatography with electrospray ionization mass spectrometry method was developed to quantitatively and qualitatively analyze 13 hydroxamate siderophores (ferrichrome, ferrirubin, ferrirhodin, ferrichrysin, ferricrocin, ferrioxamine B, D1, E and G, neocoprogen I and II, coprogen and triacetylfusarinine C). Samples were preconcentrated on‐line by a switch‐valve setup prior to analyte separation on a Kinetex C18 column. Gradient elution was performed using a mixture of an ammonium formate buffer and acetonitrile. Total analysis time including column conditioning was 20.5 min. Analytes were fragmented by applying collision‐induced dissociation, enabling structural identification by tandem mass spectrometry. Limit of detection values for the selected ion monitoring method ranged from 71 pM to 1.5 nM with corresponding values of two to nine times higher for the multiple reaction monitoring method. The liquid chromatography with mass spectrometry method resulted in a robust and sensitive quantification of hydroxamate siderophores as indicated by retention time stability, linearity, sensitivity, precision and recovery. The analytical error of the methods, assessed through random‐order, duplicate analysis of soil samples extracted with a mixture of 10 mM phosphate buffer and methanol, appears negligible in relation to between‐sample variations.
ISSN:1615-9306
1615-9314
1615-9314
DOI:10.1002/jssc.201500509