Epidermal growth factor receptor expression: predictive value for the outcome after cystectomy for bladder cancer?

• Published in: BJU international 1999-03, Vol.83 (4), p.498-503
• Main Authors: Sriplakich, S, Jahnson, S, Karlsson, M G
• Format: Article
• Language: English
• Subjects: Adult; Aged; bladder cancer; cystectomy; Cystectomy - methods; Epidermal growth factor receptor; ErbB Receptors - metabolism; Female; Humans; Immunohistochemistry - methods; Male; Middle Aged; Postoperative Care; prognosis; Retrospective Studies; Survival Analysis; Treatment Outcome; Urinary Bladder Neoplasms - metabolism; Urinary Bladder Neoplasms - surgery
• ISSN: 1464-4096; 1464-410X; 1464-410X
• DOI: 10.1046/j.1464-410x.1999.00914.x

Objective To determine whether epidermal growth factor receptor (EGFR) immunostaining of tumour cells is associated with cancer‐specific death after cystectomy for locally advanced bladder cancer. Patients and methods The hospital records of all patients treated with cystectomy for urothelial cancer of the urinary bladder between 1967 and 1992 were reviewed retrospectively. The paraffin‐embedded specimens obtained before treatment from 173 patients were processed for immunohistochemical staining, using the monoclonal antibody NCL‐EGFR (Novocastra, UK). EGFR immunostaining was considered positive if membrane staining was found in at ≥20% of tumour cells in one or more fields at ≥200 (area 0.59 mm2 ). Results Most patients (149) received preoperative irradiation and one had neoadjuvant chemotherapy. The mean observation time was 81.3 months; 63 patients (36%) had tumour recurrence within 1–80 months (mean 18.3). Positive EGFR immunostaining was found in 100 patients (58%). The proportion of T2–4 tumours was higher in those EGFR‐positive than in those EGFR‐negative. Proportional‐hazards analysis revealed that clinical stage was significantly associated with cancer‐specific death, but EGFR expression was not. Conclusion Although positive immunostaining for EGFR was more frequent in higher stages of locally advanced bladder cancer, this variable was not an independent predictor of outcome after cystectomy.