Phenolic metabolites as compliance biomarker for polyphenol intake in a randomized controlled human intervention

• Published in: Food research international 2014, Vol.63 (B), p.233-238
• Main Authors: Vetrani, Claudia, Rivellese, Angela A., Annuzzi, Giovanni, Mattila, Ismo, Meudec, Emanuelle, Hyötyläinen, Tuulia, Orešič, Matej, Aura, Anna-Marja
• Format: Article
• Language: English
• Subjects: Agricultural sciences; Biological and medical sciences; Biomarkers; Dietary polyphenol; Food and Nutrition; Food industries; Fundamental and applied biological sciences. Psychology; Human compliance; Life Sciences; Phenolic metabolites
• ISSN: 0963-9969; 1873-7145; 1873-7145
• DOI: 10.1016/j.foodres.2014.01.018

Clinical trials are needed to confirm the association between polyphenol consumption and lower incidence of chronic diseases observed in epidemiological studies. However, a reliable evaluation of polyphenol intake is complicated. An 8-week randomized controlled trial (78 subjects) was performed using two isoenergetic diets differing only for polyphenol contents. Then, urinary phenolic metabolite profiles were analysed using gas-chromatography with mass detection. Phenolic metabolites detected in 24-h-urine were hydroxylated phenolic acids with C1–C3 side chain in the group consuming polyphenol-rich diet. The intake of polyphenol-rich foods increases the excretion of phenolic metabolites in urine providing a profile, which may serve as compliance biomarker of polyphenol-rich diet. •Several precursors in foods are converted to a relatively small number of colonic microbial metabolites•Phenolic acids with C1-C3 side chain formed a profile•Metabolite profile of 24h-urine is a reliable biomarker of dietary polyphenol compliance