Separation and identification of lipid classes by normal phase LC‐ESI/MS/MS on a cyanopropyl column

In order to establish a versatile and convenient method for the analysis of lipids, electrospray ionization tandem mass spectrometry (ESI‐MS/MS) was applied to a HPLC separation on a cyanopropyl‐bonded stationary phase. A binary gradient mobile phase system consisting of hexane, toluene, methanol an...

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Bibliographic Details
Published in:European journal of lipid science and technology 2014-05, Vol.116 (5), p.653-658
Main Authors: Olsson, Petter, Holmbäck, Jan, Nilsson, Ulrika, Herslöf, Bengt
Format: Article
Language:English
Subjects:
Alkali metal ion adducts
Chromatography
Cyanopropyl
egg yolk
ESI-MS
hexane
high performance liquid chromatography
ionization
ions
Lipid classes
lysophosphatidylcholine
Mass spectrometry
methanol
Normal phase HPLC
sodium
toluene
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Summary:In order to establish a versatile and convenient method for the analysis of lipids, electrospray ionization tandem mass spectrometry (ESI‐MS/MS) was applied to a HPLC separation on a cyanopropyl‐bonded stationary phase. A binary gradient mobile phase system consisting of hexane, toluene, methanol and a stable electrospray yielding sodium adduct ions could be used to generate specific product ions in MS/MS mode. By applying the LC/ESI‐MS/MS method on an egg yolk sample, 29 different molecular species of phosphatidylethanolamines, phosphatidylcholines, and lysophosphatidylcholines could be detected within 25 min.
ISSN:1438-7697
1438-9312
1438-9312
DOI:10.1002/ejlt.201300291