Caging Polycations: Effect of Increasing Confinement on the Modes of Interaction of Spermidine 3+ With DNA Double Helices

Polyamines have important roles in the modulation of the cellular function and are ubiquitous in cells. The polyamines putrescine , spermidine , and spermine represent the most abundant organic counterions of the negatively charged DNA in the cellular nucleus. These polyamines are known to stabilize...

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Bibliographic Details
Published in:Frontiers in chemistry 2022, Vol.10, p.836994
Main Authors: Vasiliu, Tudor, Mocci, Francesca, Laaksonen, Aatto, Engelbrecht, Leon De Villiers, Perepelytsya, Sergiy
Format: Article
Language:English
Subjects:
condensation
counterion
DNA
Energiteknik
Energy Engineering
molecular dynamics
polyamine
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Summary:Polyamines have important roles in the modulation of the cellular function and are ubiquitous in cells. The polyamines putrescine , spermidine , and spermine represent the most abundant organic counterions of the negatively charged DNA in the cellular nucleus. These polyamines are known to stabilize the DNA structure and, depending on their concentration and additional salt composition, to induce DNA aggregation, which is often referred to as condensation. However, the modes of interactions of these elongated polycations with DNA and how they promote condensation are still not clear. In the present work, atomistic molecular dynamics (MD) computer simulations of two DNA fragments surrounded by spermidine (Spd ) cations were performed to study the structuring of Spd "caged" between DNA molecules. Microsecond time scale simulations, in which the parallel DNA fragments were constrained at three different separations, but allowed to rotate axially and move naturally, provided information on the conformations and relative orientations of surrounding Spm cations as a function of DNA-DNA separation. Novel geometric criteria allowed for the classification of DNA-Spd interaction modes, with special attention given to Spd conformational changes in the space between the two DNA molecules (caged Spd ). This work shows how changes in the accessible space, or confinement, around DNA affect DNA-Spd interactions, information fundamental to understanding the interactions between DNA and its counterions in environments where DNA is compacted, e.g. in the cellular nucleus.
ISSN:2296-2646
2296-2646
DOI:10.3389/fchem.2022.836994