Biogenesis of MalF and the MalFGK2 Maltose Transport Complex in Escherichia coli Requires YidC

The polytopic inner membrane protein MalF is a constituent of the MalFGK2 maltose transport complex in Escherichia coli. We have studied the biogenesis of MalF using a combination of in vivo and in vitro approaches. MalF is targeted via the SRP pathway to the Sec/YidC insertion site. Despite close p...

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Bibliographic Details
Published in:The Journal of biological chemistry 2008-06, Vol.283 (26), p.17881-17890
Main Authors: Wagner, Samuel, Pop, Ovidio, Haan, Gert-Jan, Baars, Louise, Koningstein, Gregory, Klepsch, Mirjam M., Genevaux, Pierre, Luirink, Joen, de Gier, Jan-Willem
Format: Article
Language:English
Subjects:
and Biogenesis
Biochemistry
Biokemi
Chemistry
Function
Kemi
Membrane Transport
NATURAL SCIENCES
NATURVETENSKAP
Structure
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Summary:The polytopic inner membrane protein MalF is a constituent of the MalFGK2 maltose transport complex in Escherichia coli. We have studied the biogenesis of MalF using a combination of in vivo and in vitro approaches. MalF is targeted via the SRP pathway to the Sec/YidC insertion site. Despite close proximity of nascent MalF to YidC during insertion, YidC is not required for the insertion of MalF into the membrane. However, YidC is required for the stability of MalF and the formation of the MalFGK2 maltose transport complex. Our data indicate that YidC supports the folding of MalF into a stable conformation before it is incorporated into the maltose transport complex.
ISSN:0021-9258
1083-351X
1083-351X
DOI:10.1074/jbc.M801481200