Two novel disaccharides, rutinose and methylrutinose, are involved in carbon metabolism in Datisca glomerata

• Published in: Planta 2010-02, Vol.231 (3), p.507-521
• Main Authors: Schubert, Maria, Melnikova, Anna N, Mesecke, Nikola, Zubkova, Elena K, Fortte, Rocco, Batashev, Denis R, Barth, Inga, Sauer, Norbert, Gamalei, Yuri V, Mamushina, Natalia S, Tietze, Lutz F, Voitsekhovskaja, Olga V, Pawlowski, Katharina
• Format: Article
• Language: English
• Subjects: Actinobacteria - physiology; Agriculture; Arabidopsis; Arabidopsis - metabolism; Arabidopsis Proteins - metabolism; Biological Transport; Biomedical and Life Sciences; Carbon - metabolism; Carbon sequestration; Cell membranes; Datisca; Datisca glomerata; Disaccharides; Disaccharides - chemistry; Disaccharides - isolation & purification; Disaccharides - metabolism; Ecology; Forestry; Frankia; Leaves; Life Sciences; Membrane Transport Proteins - metabolism; Mesophyll cells; Models, Biological; NATURAL SCIENCES; NATURVETENSKAP; Nitrogen Fixation; Nodules; Original; Original Article; Phloem; Plant roots; Plant Sciences; Plant veins; Plants; Plants - metabolism; Plants - microbiology; Root Nodules, Plant - metabolism; Substrate Specificity; Sucrose - metabolism; Sugar; Sugars; Symbiosis; Translocation
• ISSN: 0032-0935; 1432-2048; 1432-2048
• DOI: 10.1007/s00425-009-1049-5

Datisca glomerata forms nitrogen-fixing root nodules in symbiosis with soil actinomycetes from the genus Frankia. Analysis of sugars in roots, nodules and leaves of D. glomerata revealed the presence of two novel compounds that were identified as α-l-rhamnopyranoside-(1 → 6)-d-glucose (rutinose) and α-l-rhamnopyranoside-(1 → 6)-1-O-β-d-methylglucose (methylrutinose). Rutinose has been found previously as a/the glycoside part of several flavonoid glycosides, e.g. rutin, also of datiscin, the main flavonoid of Datisca cannabina, but had not been reported as free sugar. Time course analyses suggest that both rutinose and methylrutinose might play a role in transient carbon storage in sink organs and, to a lesser extent, in source leaves. Their concentrations show that they can accumulate in the vacuole. Rutinose, but not methylrutinose, was accepted as a substrate by the tonoplast disaccharide transporter SUT4 from Arabidopsis. In vivo ¹⁴C-labeling and the study of uptake of exogenous sucrose and rutinose from the leaf apoplast showed that neither rutinose nor methylrutinose appreciably participate in phloem translocation of carbon from source to sink organs, despite rutinose being found in the apoplast at significant levels. A model for sugar metabolism in D. glomerata is presented.