Screening and determination of drugs in human saliva utilizing microextraction by packed sorbent and liquid chromatography-tandem mass spectrometry

ABSTRACT This study presents a new method for collecting and handling saliva samples using an automated analytical microsyringe and microextraction by packed syringe (MEPS). The screening and determination of lidocaine in human saliva samples utilizing MEPS and liquid chromatography–tandem mass spec...

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Bibliographic Details
Published in:Biomedical chromatography 2013-09, Vol.27 (9), p.1188-1191
Main Authors: Abdel Rehim, Abbi, Abdel Rehim, Mohamed
Format: Article
Language:English
Subjects:
Chromatography, Liquid - methods
eVol
Humans
LC-MS/MS
lidocaine
Lidocaine - analysis
Lidocaine - isolation & purification
MEPS
Models, Chemical
Pharmaceutical Preparations - analysis
Pharmaceutical Preparations - isolation & purification
Saliva - chemistry
saliva samples
Solid Phase Microextraction - methods
Tandem Mass Spectrometry - methods
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Summary:ABSTRACT This study presents a new method for collecting and handling saliva samples using an automated analytical microsyringe and microextraction by packed syringe (MEPS). The screening and determination of lidocaine in human saliva samples utilizing MEPS and liquid chromatography–tandem mass spectrometry (LC‐MS/MS) were carried out. An exact volume of saliva could be collected. The MEPS C8‐cartridge could be used for 50 extractions before it was discarded. The extraction recovery was about 60%. The pharmacokinetic curve of lidocaine in saliva using MEPS‐LC‐MS/MS is reported. Copyright © 2013 John Wiley & Sons, Ltd.
ISSN:0269-3879
1099-0801
1099-0801
DOI:10.1002/bmc.2925