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Screening and determination of drugs in human saliva utilizing microextraction by packed sorbent and liquid chromatography-tandem mass spectrometry

ABSTRACT This study presents a new method for collecting and handling saliva samples using an automated analytical microsyringe and microextraction by packed syringe (MEPS). The screening and determination of lidocaine in human saliva samples utilizing MEPS and liquid chromatography–tandem mass spec...

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Published in:Biomedical chromatography 2013-09, Vol.27 (9), p.1188-1191
Main Authors: Abdel Rehim, Abbi, Abdel Rehim, Mohamed
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Language:English
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cited_by cdi_FETCH-LOGICAL-c4875-6609da80ecf9676d0217d2addde350665ac7d82a5e833ccec945849cee8fde0e3
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Abdel Rehim, Mohamed
description ABSTRACT This study presents a new method for collecting and handling saliva samples using an automated analytical microsyringe and microextraction by packed syringe (MEPS). The screening and determination of lidocaine in human saliva samples utilizing MEPS and liquid chromatography–tandem mass spectrometry (LC‐MS/MS) were carried out. An exact volume of saliva could be collected. The MEPS C8‐cartridge could be used for 50 extractions before it was discarded. The extraction recovery was about 60%. The pharmacokinetic curve of lidocaine in saliva using MEPS‐LC‐MS/MS is reported. Copyright © 2013 John Wiley & Sons, Ltd.
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identifier ISSN: 0269-3879
ispartof Biomedical chromatography, 2013-09, Vol.27 (9), p.1188-1191
issn 0269-3879
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1099-0801
language eng
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source Wiley
subjects Chromatography, Liquid - methods
eVol
Humans
LC-MS/MS
lidocaine
Lidocaine - analysis
Lidocaine - isolation & purification
MEPS
Models, Chemical
Pharmaceutical Preparations - analysis
Pharmaceutical Preparations - isolation & purification
Saliva - chemistry
saliva samples
Solid Phase Microextraction - methods
Tandem Mass Spectrometry - methods
title Screening and determination of drugs in human saliva utilizing microextraction by packed sorbent and liquid chromatography-tandem mass spectrometry
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