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Screening and determination of drugs in human saliva utilizing microextraction by packed sorbent and liquid chromatography-tandem mass spectrometry
ABSTRACT This study presents a new method for collecting and handling saliva samples using an automated analytical microsyringe and microextraction by packed syringe (MEPS). The screening and determination of lidocaine in human saliva samples utilizing MEPS and liquid chromatography–tandem mass spec...
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Published in: | Biomedical chromatography 2013-09, Vol.27 (9), p.1188-1191 |
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container_issue | 9 |
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container_title | Biomedical chromatography |
container_volume | 27 |
creator | Abdel Rehim, Abbi Abdel Rehim, Mohamed |
description | ABSTRACT
This study presents a new method for collecting and handling saliva samples using an automated analytical microsyringe and microextraction by packed syringe (MEPS). The screening and determination of lidocaine in human saliva samples utilizing MEPS and liquid chromatography–tandem mass spectrometry (LC‐MS/MS) were carried out. An exact volume of saliva could be collected. The MEPS C8‐cartridge could be used for 50 extractions before it was discarded. The extraction recovery was about 60%. The pharmacokinetic curve of lidocaine in saliva using MEPS‐LC‐MS/MS is reported. Copyright © 2013 John Wiley & Sons, Ltd. |
doi_str_mv | 10.1002/bmc.2925 |
format | article |
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This study presents a new method for collecting and handling saliva samples using an automated analytical microsyringe and microextraction by packed syringe (MEPS). The screening and determination of lidocaine in human saliva samples utilizing MEPS and liquid chromatography–tandem mass spectrometry (LC‐MS/MS) were carried out. An exact volume of saliva could be collected. The MEPS C8‐cartridge could be used for 50 extractions before it was discarded. The extraction recovery was about 60%. The pharmacokinetic curve of lidocaine in saliva using MEPS‐LC‐MS/MS is reported. Copyright © 2013 John Wiley & Sons, Ltd.</description><identifier>ISSN: 0269-3879</identifier><identifier>ISSN: 1099-0801</identifier><identifier>EISSN: 1099-0801</identifier><identifier>DOI: 10.1002/bmc.2925</identifier><identifier>PMID: 23629973</identifier><language>eng</language><publisher>England: Blackwell Publishing Ltd</publisher><subject>Chromatography, Liquid - methods ; eVol ; Humans ; LC-MS/MS ; lidocaine ; Lidocaine - analysis ; Lidocaine - isolation & purification ; MEPS ; Models, Chemical ; Pharmaceutical Preparations - analysis ; Pharmaceutical Preparations - isolation & purification ; Saliva - chemistry ; saliva samples ; Solid Phase Microextraction - methods ; Tandem Mass Spectrometry - methods</subject><ispartof>Biomedical chromatography, 2013-09, Vol.27 (9), p.1188-1191</ispartof><rights>Copyright © 2013 John Wiley & Sons, Ltd.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4875-6609da80ecf9676d0217d2addde350665ac7d82a5e833ccec945849cee8fde0e3</citedby><cites>FETCH-LOGICAL-c4875-6609da80ecf9676d0217d2addde350665ac7d82a5e833ccec945849cee8fde0e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,780,784,885,27923,27924</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23629973$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://urn.kb.se/resolve?urn=urn:nbn:se:su:diva-93554$$DView record from Swedish Publication Index$$Hfree_for_read</backlink></links><search><creatorcontrib>Abdel Rehim, Abbi</creatorcontrib><creatorcontrib>Abdel Rehim, Mohamed</creatorcontrib><title>Screening and determination of drugs in human saliva utilizing microextraction by packed sorbent and liquid chromatography-tandem mass spectrometry</title><title>Biomedical chromatography</title><addtitle>Biomed. Chromatogr</addtitle><description>ABSTRACT
This study presents a new method for collecting and handling saliva samples using an automated analytical microsyringe and microextraction by packed syringe (MEPS). The screening and determination of lidocaine in human saliva samples utilizing MEPS and liquid chromatography–tandem mass spectrometry (LC‐MS/MS) were carried out. An exact volume of saliva could be collected. The MEPS C8‐cartridge could be used for 50 extractions before it was discarded. The extraction recovery was about 60%. The pharmacokinetic curve of lidocaine in saliva using MEPS‐LC‐MS/MS is reported. Copyright © 2013 John Wiley & Sons, Ltd.</description><subject>Chromatography, Liquid - methods</subject><subject>eVol</subject><subject>Humans</subject><subject>LC-MS/MS</subject><subject>lidocaine</subject><subject>Lidocaine - analysis</subject><subject>Lidocaine - isolation & purification</subject><subject>MEPS</subject><subject>Models, Chemical</subject><subject>Pharmaceutical Preparations - analysis</subject><subject>Pharmaceutical Preparations - isolation & purification</subject><subject>Saliva - chemistry</subject><subject>saliva samples</subject><subject>Solid Phase Microextraction - methods</subject><subject>Tandem Mass Spectrometry - methods</subject><issn>0269-3879</issn><issn>1099-0801</issn><issn>1099-0801</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><recordid>eNp1kc1u1DAUhS0EokNB4gmQl0goxbETJ162KRSk8iOVn6Xl2DczpnGc2g5teA1emExnGFas7uJ851vcg9DznJzkhNDXrdMnVNDyAVrlRIiM1CR_iFaEcpGxuhJH6EmMPwghgtPqMTqijFMhKrZCv690ABjssMZqMNhAguDsoJL1A_YdNmFaR2wHvJmcGnBUvf2p8JRsb39tS87q4OEuBaXvK-2MR6WvweDoQwtDutf29mayButN8E4lvw5q3MxZWiJw2KkYcRxBpyWFFOan6FGn-gjP9vcYfX375kvzLrv8dPG-Ob3MdFFXZcY5EUbVBHQneMUNoXllqDLGACsJ56XSlampKqFmTGvQoijrQmiAujNAgB2jVztvvIVxauUYrFNhll5ZeW6_nUof1jJOUrCyLBb65Y4eg7-ZICbpbNTQ92oAP0WZF3nNeEEo_Ycuv4kxQHdQ50RuB5PLYHI72IK-2Fun1oE5gH8XWoBsB9zaHub_iuTZh2Yv3PM2Jrg78CpcS16xqpTfP17I5upMFORzIzn7A5-6svk</recordid><startdate>201309</startdate><enddate>201309</enddate><creator>Abdel Rehim, Abbi</creator><creator>Abdel Rehim, Mohamed</creator><general>Blackwell Publishing Ltd</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>ADTPV</scope><scope>AOWAS</scope><scope>DG7</scope></search><sort><creationdate>201309</creationdate><title>Screening and determination of drugs in human saliva utilizing microextraction by packed sorbent and liquid chromatography-tandem mass spectrometry</title><author>Abdel Rehim, Abbi ; Abdel Rehim, Mohamed</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4875-6609da80ecf9676d0217d2addde350665ac7d82a5e833ccec945849cee8fde0e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Chromatography, Liquid - methods</topic><topic>eVol</topic><topic>Humans</topic><topic>LC-MS/MS</topic><topic>lidocaine</topic><topic>Lidocaine - analysis</topic><topic>Lidocaine - isolation & purification</topic><topic>MEPS</topic><topic>Models, Chemical</topic><topic>Pharmaceutical Preparations - analysis</topic><topic>Pharmaceutical Preparations - isolation & purification</topic><topic>Saliva - chemistry</topic><topic>saliva samples</topic><topic>Solid Phase Microextraction - methods</topic><topic>Tandem Mass Spectrometry - methods</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Abdel Rehim, Abbi</creatorcontrib><creatorcontrib>Abdel Rehim, Mohamed</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>SwePub</collection><collection>SwePub Articles</collection><collection>SWEPUB Stockholms universitet</collection><jtitle>Biomedical chromatography</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Abdel Rehim, Abbi</au><au>Abdel Rehim, Mohamed</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Screening and determination of drugs in human saliva utilizing microextraction by packed sorbent and liquid chromatography-tandem mass spectrometry</atitle><jtitle>Biomedical chromatography</jtitle><addtitle>Biomed. Chromatogr</addtitle><date>2013-09</date><risdate>2013</risdate><volume>27</volume><issue>9</issue><spage>1188</spage><epage>1191</epage><pages>1188-1191</pages><issn>0269-3879</issn><issn>1099-0801</issn><eissn>1099-0801</eissn><abstract>ABSTRACT
This study presents a new method for collecting and handling saliva samples using an automated analytical microsyringe and microextraction by packed syringe (MEPS). The screening and determination of lidocaine in human saliva samples utilizing MEPS and liquid chromatography–tandem mass spectrometry (LC‐MS/MS) were carried out. An exact volume of saliva could be collected. The MEPS C8‐cartridge could be used for 50 extractions before it was discarded. The extraction recovery was about 60%. The pharmacokinetic curve of lidocaine in saliva using MEPS‐LC‐MS/MS is reported. Copyright © 2013 John Wiley & Sons, Ltd.</abstract><cop>England</cop><pub>Blackwell Publishing Ltd</pub><pmid>23629973</pmid><doi>10.1002/bmc.2925</doi><tpages>4</tpages></addata></record> |
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language | eng |
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source | Wiley |
subjects | Chromatography, Liquid - methods eVol Humans LC-MS/MS lidocaine Lidocaine - analysis Lidocaine - isolation & purification MEPS Models, Chemical Pharmaceutical Preparations - analysis Pharmaceutical Preparations - isolation & purification Saliva - chemistry saliva samples Solid Phase Microextraction - methods Tandem Mass Spectrometry - methods |
title | Screening and determination of drugs in human saliva utilizing microextraction by packed sorbent and liquid chromatography-tandem mass spectrometry |
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