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Determination of methotrexate in spiked human blood serum using multi-frequency electrochemical immittance spectroscopy and multivariate data analysis
This article describes an attempt to develop a sensor based on multi-frequency immittance spectroscopy for the determination of methotrexate (MTX) in blood serum using gold electrodes modified with antibodies. The attachment of antibodies was monitored with electrochemical immittance spectroscopy (E...
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Published in: | Analytica chimica acta 2017-09, Vol.987, p.15-24 |
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Main Authors: | , , , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Get full text |
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Summary: | This article describes an attempt to develop a sensor based on multi-frequency immittance spectroscopy for the determination of methotrexate (MTX) in blood serum using gold electrodes modified with antibodies. The attachment of antibodies was monitored with electrochemical immittance spectroscopy (EIS) and X-ray photoelectron spectroscopy (XPS). The EIS measurements of MTX resulted in a data matrix of size 39 × 55. The data were analysed using multivariate data analysis and showed a concentration dependence and time dependence that could be separated. This allowed the calculation of a multivariate calibration model. The model showed good linear behavior on a logarithmic scale offering a detection limit of 5 × 10−12 mol L−1.
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•Determination of methotrexate (MTX) in spiked blood serum using a biosensor; gold electrodes modified with antibody.•Electrode surface modification was performed by introducing reagents while the electrodes were mounted in a flow cell.•The electrode modification was monitored using electrochemical immittance spectroscopy and X-ray photoelectron spectroscopy.•The method offered a wide linear working range with 8 orders of magnitude and a detection limit of 5 × 10-12 mol L-1. |
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ISSN: | 0003-2670 1873-4324 1873-4324 |
DOI: | 10.1016/j.aca.2017.08.034 |