Crystal structures of OrfX2 and P47 from a Botulinum neurotoxin OrfX‐type gene cluster

Botulinum neurotoxins are highly toxic substances and are all encoded together with one of two alternative gene clusters, the HA or the OrfX gene cluster. Very little is known about the function and structure of the proteins encoded in the OrfX gene cluster, which in addition to the toxin contains f...

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Bibliographic Details
Published in:FEBS letters 2017-11, Vol.591 (22), p.3781-3792
Main Authors: Gustafsson, Robert, Berntsson, Ronnie P‐A, Martínez‐Carranza, Markel, El Tekle, Geniver, Odegrip, Richard, Johnson, Eric A., Stenmark, Pål
Format: Article
Language:English
Subjects:
Bacterial Proteins - chemistry
Bacterial Proteins - genetics
Bacterial Proteins - metabolism
Biochemistry
biokemi
botulinum neurotoxin
Cloning, Molecular
Clostridium botulinum - chemistry
Clostridium botulinum - genetics
Clostridium botulinum - metabolism
Crystallography
Crystallography, X-Ray
gene cluster
Models, Molecular
Multigene Family
Phosphatidylinositols - metabolism
Protein Binding
Protein Conformation
TULIP
X-ray
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Summary:Botulinum neurotoxins are highly toxic substances and are all encoded together with one of two alternative gene clusters, the HA or the OrfX gene cluster. Very little is known about the function and structure of the proteins encoded in the OrfX gene cluster, which in addition to the toxin contains five proteins (OrfX1, OrfX2, OrfX3, P47, and NTNH). We here present the structures of OrfX2 and P47, solved to 2.1 and 1.8 Å, respectively. We show that they belong to the TULIP protein superfamily, which are often involved in lipid binding. OrfX1 and OrfX2 were both found to bind phosphatidylinositol lipids.
ISSN:0014-5793
1873-3468
1873-3468
DOI:10.1002/1873-3468.12889