Identification of factors that promote biogenesis of tRNA CGA Ser

• Published in: RNA biology 2018, Vol.15 (10), p.1286-1294
• Main Authors: Xu, Fu, Zhou, Yang, Byström, Anders S, Johansson, Marcus J O
• Format: Article
• Language: English
• Subjects: Adenosine Triphosphatases - genetics; Alkyl and Aryl Transferases - genetics; Carrier Proteins - genetics; modified nucleosides; Mutation; Nuclear Pore Complex Proteins - genetics; Oxidoreductases - genetics; Pol1 Transcription Initiation Complex Proteins - genetics; Protein Biosynthesis; RNA Precursors - biosynthesis; RNA Precursors - genetics; RNA, Transfer - biosynthesis; RNA, Transfer - genetics; Saccharomyces cerevisiae - genetics; Saccharomyces cerevisiae Proteins - genetics; sup61; TATA-Binding Protein Associated Factors - genetics; tRNA maturation; tRNA Methyltransferases - genetics; tRNA modification; tRNASer
• ISSN: 1547-6286; 1555-8584; 1555-8584
• DOI: 10.1080/15476286.2018.1526539

A wide variety of factors are required for the conversion of pre-tRNA molecules into the mature tRNAs that function in translation. To identify factors influencing tRNA biogenesis, we previously performed a screen for strains carrying mutations that induce lethality when combined with a sup61-T47:2C allele, encoding a mutant form of [Formula: see text]. Analyzes of two complementation groups led to the identification of Tan1 as a protein involved in formation of the modified nucleoside N -acetylcytidine (ac C) in tRNA and Bud13 as a factor controlling the levels of ac C by promoting TAN1 pre-mRNA splicing. Here, we describe the remaining complementation groups and show that they include strains with mutations in genes for known tRNA biogenesis factors that modify (DUS2, MOD5 and TRM1), transport (LOS1), or aminoacylate (SES1) [Formula: see text]. Other strains carried mutations in genes for factors involved in rRNA/mRNA synthesis (RPA49, RRN3 and MOT1) or magnesium uptake (ALR1). We show that mutations in not only DUS2, LOS1 and SES1 but also in RPA49, RRN3 and MOT1 cause a reduction in the levels of the altered [Formula: see text]. These results indicate that Rpa49, Rrn3 and Mot1 directly or indirectly influence [Formula: see text] biogenesis.