An Arabidopsis thaliana protein homologous to cyanobacterial high-light-inducible proteins

An Arabidopsis thaliana cDNA clone encoding a novel 110 amino acid thylakoid protein has been sequenced. The in vitro synthesized protein is taken up by intact chloroplasts, inserted into the thylakoid membrane and the transit peptide is cleaved off during this process. The mature protein is predict...

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Bibliographic Details
Published in:Plant molecular biology 2000-01, Vol.42 (2), p.345-351
Main Authors: Jansson, S, Andersson, J, Kim, S J, Jackowski, G
Format: Article
Language:English
Subjects:
Algae
Amino Acid Sequence
Amino acids
Arabidopsis - genetics
Arabidopsis - radiation effects
Arabidopsis thaliana
Bacteria
Bacterial Proteins
Base Sequence
Chloroplasts - metabolism
Circadian Rhythm
DNA, Complementary - chemistry
DNA, Complementary - genetics
ELIP
EST
Gene Expression Regulation, Plant - radiation effects
Genes
high light
high-light-inducible proteins
HLIP
LHC protein
Light
Light-Harvesting Protein Complexes
Membranes
Molecular Sequence Data
Photosynthetic Reaction Center Complex Proteins - genetics
Plant Proteins - genetics
Plant Proteins - pharmacokinetics
Protein Precursors - pharmacokinetics
Proteins
RNA, Plant - genetics
RNA, Plant - metabolism
Sequence Alignment
Sequence Analysis, DNA
Sequence Homology, Amino Acid
Space life sciences
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Summary:An Arabidopsis thaliana cDNA clone encoding a novel 110 amino acid thylakoid protein has been sequenced. The in vitro synthesized protein is taken up by intact chloroplasts, inserted into the thylakoid membrane and the transit peptide is cleaved off during this process. The mature protein is predicted to contain 69 amino acids, to form one membrane-spanning alpha-helix and to have its N-terminus at the stromal side of the thylakoid membrane. The protein showed similarity to the LHC, ELIP and PsbS proteins of higher plants, but more pronounced to the high-light-inducible proteins (HLIPs) of cyanobacteria and red algae, to which no homologue previously has been detected in higher plants. As for HLIP and ELIP, high light increases the mRNA levels of the corresponding gene. Sequence comparisons indicate that the protein may bind chlorophyll and form dimers in the thylakoid membrane. The level of expression of the protein seems to be far lower than that of normal PSI and PSII subunits.
ISSN:0167-4412
1573-5028
1573-5028
DOI:10.1023/A:1006365213954