The role of mitochondrial electron transport during photosynthetic induction: A study with barley (Hordeum vulgare) protoplasts incubated with rotenone and oligomycin

Mitochondrial contribution to photosynthetic metabolism during photosynthetic induction was investigated in protoplasts from barley leaves (Hordeum vulgare L. cv. Gunilla, Svalöf) by using an inhibitor of mitochondrial Complex I (rotenone) and an inhibitor of the mitochondrial ATPase (oligomycin). B...

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Bibliographic Details
Published in:Physiologia plantarum 1998-11, Vol.104 (3), p.431-439
Main Authors: Igamberdiev, A.U, Hurry, V, Kroemer, S, Gardestroem, P. (Umeaa Univ. (Sweden))
Format: Article
Language:English
Subjects:
Agronomy. Soil science and plant productions
Biological and medical sciences
Economic plant physiology
FEUILLE
FLUORESCENCE
FLUORESCENCIA
FOTOSINTESIS
Fundamental and applied biological sciences. Psychology
HOJAS
HORDEUM VULGARE
LEAVES
LIGHT
LUMIERE
LUZ
MALATE DEHYDROGENASE
MALATE DESHYDROGENASE
MALATO DESHIDROGENASA
Metabolism
MITOCHONDRIA
MITOCHONDRIE
MITOCONDRIA
Net assimilation, photosynthesis, carbon metabolism. Photorespiration, respiration, fermentation (anoxia, hypoxia)
Nutrition. Photosynthesis. Respiration. Metabolism
PHOTOSYNTHESE
PHOTOSYNTHESIS
Photosynthesis, respiration. Anabolism, catabolism
Plant physiology and development
PROTOPLASTE
PROTOPLASTOS
PROTOPLASTS
RESPIRACION
RESPIRATION
ROTENONA
ROTENONE
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Summary:Mitochondrial contribution to photosynthetic metabolism during photosynthetic induction was investigated in protoplasts from barley leaves (Hordeum vulgare L. cv. Gunilla, Svalöf) by using an inhibitor of mitochondrial Complex I (rotenone) and an inhibitor of the mitochondrial ATPase (oligomycin). Both inhibitors increased the lag phase of photosynthetic induction after the transition of protoplasts from darkness to light. This effect was not observed with broken protoplasts or isolated chloroplasts. Using the method of rapid fractionation of protoplasts it was shown that the delay in photosynthetic induction was accompanied by a decrease in ATP/ADP ratios of the cytosol and mitochondria, whereas the ratio in chloroplasts was not affected. A delay in activation of chloroplastidic NADP‐dependent malate dehydrogenase (EC 1.1.1.82) was observed in the presence of either inhibitor. A delay was also observed in the rise of photochemical quenching of chlorophyll fluorescence in the presence of rotenone or oligomycin during photosynthetic induction. The results indicate that during the transition from dark to light the mitochondrial electron transport chain and its Complex I participate in the reoxidation of excessive redox equivalents from photosynthetic electron transport.
ISSN:0031-9317
1399-3054
1399-3054
DOI:10.1034/j.1399-3054.1998.1040319.x