Reliability of near-infrared spectroscopy for measuring forearm and shoulder oxygenation in healthy males and females

• Published in: European journal of applied physiology 2012-07, Vol.112 (7), p.2703-2715
• Main Authors: Crenshaw, Albert G., Elcadi, Guilherme H., Hellstrom, Fredrik, Mathiassen, Svend Erik
• Format: Article
• Language: English
• Subjects: Adult; Arm - physiology; Between-subject variability; Biological and medical sciences; Biomedical and Life Sciences; Biomedicine; Blood; Female; Fundamental and applied biological sciences. Psychology; Hemoglobin; Human Physiology; Humans; Intraclass correlation; Isometric Contraction - physiology; Limits of agreement; Male; Muscle, Skeletal - physiology; Near-infrared spectroscopy; Occupational Medicine/Industrial Medicine; Original Article; Oximetry - methods; Oxygen - metabolism; Oxygen Consumption - physiology; Oxygenation saturation; Reproducibility of Results; Sensitivity and Specificity; Shoulder - physiology; Spectroscopy, Near-Infrared - methods; Spectrum analysis; Sports Medicine; Variables; Vertebrates: body movement. Posture. Locomotion. Flight. Swimming. Physical exercise. Rest. Sports; Within-subject variability; Young Adult
• ISSN: 1439-6319; 1439-6327; 1439-6327
• DOI: 10.1007/s00421-011-2244-1

This study determined the day-to-day reliability of NIRS-derived oxygenation responses (∆StO 2 %) for isometric contractions and for cuff occlusion. Twenty-four subjects (12 males and 12 females) were tested for 2 days (4–6 days interval). Variables generated were: (1) ∆StO 2 % for isometric contractions (10, 30, 50 and 70% MVC) for descending trapezius (TD) and extensor carpi radialis (ECR) muscles; (2) slope changes in total haemoglobin (HbTslope) and deoxyhaemoglobin (HHbslope) for the ECR using upper arm venous (VO, 50 mmHg) and arterial occlusion (AO, 250 mmHg); (3) recovery slopes (Rslope) for oxygen saturation (StO 2 ) following isometric contractions and AO. For each variable, an intraclass correlation (ICC) was calculated to assess the ability to differentiate between subjects, and limits of agreement (LOA) were computed to assess day-to-day consistency of the measurement. ICCs for ΔStO 2 % were lowest at 10% MVC for both ECR (0.58) and TD (0.55), and highest at 30% MVC for ECR (0.95) and at 70% MVC for TD (0.79). For both muscles, LOA for ΔStO 2 % was lowest at 10% and highest at 50 and 70% MVC. ICC for HbTslope was 0.17. For HHbslope ICC was higher for AO (0.83) than for VO (0.73), and LOA was lower for AO. For the ECR Rslope ICCs ranged from 0.88 to 0.90 for contraction, but was lower for AO (0.33); LOA was lowest at 70% MVC. For trapezius Rslope ICCs ranged from 0.63 to 0.73 and LOA was lowest at 30% MVC. For this study, establishing reliability data for the ECR and TD and including variables commonly reported are expected to have meaning for future NIRS studies of work-related upper-extremity pain as well as for other NIRS research and clinical applications.