Effect of ion adsorption on CEC separation of small molecules using hypercrosslinked porous polymer monolithic capillary columns

• Published in: Journal of separation science 2012-06, Vol.35 (12), p.1502-1506
• Main Authors: Chen, Xiao-Jia, Dinh, Ngoc Phuoc, Zhao, Jing, Wang, Yi-Tao, Li, Shao-Ping, Svec, Frantisek
• Format: Article
• Language: English
• Subjects: Adsorption; Analytical chemistry; Benzene - chemistry; Buffer solutions; Capillarity; Capillary electrochromatography; Capillary Electrochromatography - instrumentation; Capillary Electrochromatography - methods; Chemistry; Chlorides; Chromatographic methods and physical methods associated with chromatography; Chromatography; Exact sciences and technology; Hypercrosslinking; Ion adsorption; Ions; Molecular chemistry; Other chromatographic methods; Polymer monolith; Polymers; Polymers - chemistry; Polystyrene resins; Porosity; Resins, Synthetic - chemistry; Separation; Surface area; Surface chemistry
• ISSN: 1615-9306; 1615-9314; 1615-9314
• DOI: 10.1002/jssc.201200138

Both poly(styrene‐co‐vinylbenzyl chloride‐co‐divinylbenzene) and poly(4‐methylstyrene‐co‐vinylbenzyl chloride‐co‐divinylbenzene) monolithic columns have been hypercrosslinked and for the first time used to achieve capillary electrochromatographic separations. Although these columns do not contain ionizable functionalities, electroosmotic flow was observed due to adsorption of ions from a buffer solution contained in the mobile phase on the surface of the hydrophobic polymer. An increase of more than one order of magnitude was observed with the use of both monolithic polymers. The hypercrosslinking reaction creates a large surface area thus enabling adsorption of a much larger number of ions. Alkylbenzenes were successfully separated using the hypercrosslinked monolithic columns.