NMR Spectroscopic Determination of the Solution Structure of a Branched Nucleic Acid from Residual Dipolar Couplings by Using Isotopically Labeled Nucleotides

Only a small set of magnetic‐field‐induced residual dipolar couplings is required to determine the global structure of branched nucleic acids. This is demonstrated for the example of the Holliday junction (shown schematically) after 13C labeling in the ribose of specific thymine residues. Accurate c...

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Bibliographic Details
Published in:Angewandte Chemie International Edition 2004-01, Vol.43 (2), p.187-192
Main Authors: van Buuren, Bernd N. M., Schleucher, Jürgen, Wittmann, Valentin, Griesinger, Christian, Schwalbe, Harald, Wijmenga, Sybren S.
Format: Article
Language:English
Subjects:
Holliday junctions
Isotopes
isotopic labeling
NMR spectroscopy
Nuclear Magnetic Resonance, Biomolecular - methods
Nucleic Acid Conformation
nucleic acids
Nucleic Acids - chemistry
Nucleotides - chemistry
Solutions
structure elucidation
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Summary:Only a small set of magnetic‐field‐induced residual dipolar couplings is required to determine the global structure of branched nucleic acids. This is demonstrated for the example of the Holliday junction (shown schematically) after 13C labeling in the ribose of specific thymine residues. Accurate calculation of the magnetic‐field‐induced alignment tensor for any nucleic acid conformation allows the reduction in the required number of parameters.
ISSN:1433-7851
1521-3773
DOI:10.1002/anie.200351632