Glucose transporter‐1 expression in renal cell carcinoma and its correlation with hypoxia inducible factor‐1α

• Published in: BJU international 2008-02, Vol.101 (4), p.480-484
• Main Authors: Lidgren, Anders, Bergh, Anders, Grankvist, Kjell, Rasmuson, Torgny, Ljungberg, Börje
• Format: Article
• Language: English
• Subjects: Adult; Aged; Aged, 80 and over; Biological and medical sciences; Biomarkers, Tumor - metabolism; Carcinoma, Renal Cell - metabolism; Carcinoma, Renal Cell - pathology; Feasibility Studies; Female; Glucose Transporter Type 1 - metabolism; glucose transporter-1; Humans; hypoxia inducible factor 1 alpha; hypoxia inducible factor 1α; Hypoxia-Inducible Factor 1, alpha Subunit - metabolism; Immunohistochemistry; Kidney Neoplasms - metabolism; Kidney Neoplasms - pathology; Kidneys; Male; Medical sciences; Microarray Analysis; Middle Aged; Neoplasm Staging; Nephrology. Urinary tract diseases; Prognosis; RCC; tissue microarray; Tumors of the urinary system
• ISSN: 1464-4096; 1464-410X; 1464-410X
• DOI: 10.1111/j.1464-410X.2007.07238.x

OBJECTIVE To evaluate transcription factor hypoxia inducible factor‐1α (HIF‐1α) activity, by analysing a target gene for HIF‐1α, glucose transporter‐1 (GLUT‐1), using a tissue microarray (TMA) in different types of renal cell carcinoma (RCC, a tumour with a variable clinical course, partly due to angiogenic activity), as angiogenesis is important for tumour progression and metastatic spread, and is activated by hypoxia. PATIENTS AND METHODS GLUT‐1 and HIF‐1α expressions were semiquantitatively analysed using immunohistological staining of a prepared TMA, using samples from 187 patients, including 148 with conventional, 26 with papillary and 13 with chromophobe RCC. RESULTS GLUT‐1 staining was found mainly in the cytoplasm. The tumours were subdivided into GLUT −1LOW and GLUT‐1HIGH, based on staining intensity. There was a significant difference in GLUT‐1 expression between RCC types (P