Resveratrol regulates the expression of LXR-α in human macrophages

The naturally occurring polyphenol resveratrol has been associated with the beneficial effects of red wine consumption on cardiovascular disease and shown to inhibit atherosclerosis in animal models. To determine if resveratrol affects the expression of genes that control lipid homeostasis in human...

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Bibliographic Details
Published in:Biochemical and biophysical research communications 2006-09, Vol.348 (3), p.1047-1054
Main Authors: Sevov, Marie, Elfineh, Lioudmila, Cavelier, Lucia B.
Format: Article
Language:English
Subjects:
Cell Line, Tumor
Chromatin immunoprecipitation
DNA-Binding Proteins - biosynthesis
DNA-Binding Proteins - genetics
Gene Expression Regulation - drug effects
Gene regulation
Humans
Lipid Metabolism - drug effects
Lipid Metabolism - genetics
Liver X Receptors
LXR-alpha
LXR-α
Macrophages
Macrophages - drug effects
Macrophages - metabolism
MEDICIN
MEDICINE
Orphan Nuclear Receptors
Receptors, Cytoplasmic and Nuclear - biosynthesis
Receptors, Cytoplasmic and Nuclear - genetics
Resveratrol
RNA, Messenger - biosynthesis
Stilbenes - pharmacology
THP-1
Up-Regulation - drug effects
Up-Regulation - genetics
Vitaceae
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Summary:The naturally occurring polyphenol resveratrol has been associated with the beneficial effects of red wine consumption on cardiovascular disease and shown to inhibit atherosclerosis in animal models. To determine if resveratrol affects the expression of genes that control lipid homeostasis in human macrophages, we measured expression changes in the LXR-α pathway, crucial to cholesterol efflux, and in genes that mediate lipoprotein uptake. Resveratrol treatment of THP-1 macrophages induced LXR-α at mRNA and protein levels. Increased recruitment of RNA polymerase II to the LXR-α promoter suggested that up-regulation was at least partly mediated by transcriptional mechanisms. Resveratrol also induced LXR-α in human monocyte-derived macrophages together with elevated ABCA1 and ABCG1 mRNA levels. Moreover, resveratrol repressed the expression of the lipid uptake genes LPL and SR-AII. The ability of resveratrol to modulate expression of the genes involved in lipid uptake and efflux suggests that polyphenols can potentially limit cholesterol accumulation in human macrophages.
ISSN:0006-291X
1090-2104
1090-2104
DOI:10.1016/j.bbrc.2006.07.155