Quantitative Overview of N 2 Fixation in Nostoc punctiforme ATCC 29133 through Cellular Enrichments and iTRAQ Shotgun Proteomics

Nostoc punctiforme ATCC 29133 is a photoautotrophic cyanobacterium with the capacity to fix atmospheric N 2 . Its ability to mediate this process is similar to that described for Nostoc sp. PCC 7120, where vegetative cells differentiate into heterocysts. Quantitative proteomic investigations at both...

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Bibliographic Details
Published in:Journal of proteome research 2009-01, Vol.8 (1), p.187-198
Main Authors: Ow, Saw Yen, Noirel, Josselin, Cardona, Tanai, Taton, Arnaud, Lindblad, Peter, Stensjö, Karin, Wright, Phillip C.
Format: Article
Language:English
Subjects:
Biochemistry
Biokemi
Chemistry
Dinitrogen Fixation
Heterocysts
iTRAQ
Kemi
NATURAL SCIENCES
NATURVETENSKAP
Nostoc punctiforme ATCC 29133
Nostoc sp PCC 73102
Shotgun proteomics
Tandem-MS
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Summary:Nostoc punctiforme ATCC 29133 is a photoautotrophic cyanobacterium with the capacity to fix atmospheric N 2 . Its ability to mediate this process is similar to that described for Nostoc sp. PCC 7120, where vegetative cells differentiate into heterocysts. Quantitative proteomic investigations at both the filament level and the heterocyst level are presented using isobaric tagging technology (iTRAQ), with 721 proteins at the 95% confidence interval quantified across both studies. Observations from both experiments yielded findings confirmatory of both transcriptional studies, and published Nostoc sp. PCC 7120 iTRAQ data. N. punctiforme exhibits similar metabolic trends, though changes in a number of metabolic pathways are less pronounced than in Nostoc sp. PCC 7120. Results also suggest a number of proteins that may benefit from future investigations. These include ATP dependent Zn-proteases, N-reserve degraders and also redox balance proteins. Complementary proteomic data sets from both organisms present key precursor knowledge that is important for future cyanobacterial biohydrogen research.
ISSN:1535-3893
1535-3907
1535-3907
DOI:10.1021/pr800285v