Pancreatic islet blood flow during euglycaemic, hyperinsulinaemic clamp in anaesthetized rats

• Published in: Acta Physiologica 2007-04, Vol.189 (4), p.319-324
• Main Authors: Jansson, L., Andersson, A., Bodin, B., Källskog, Ö.
• Format: Article
• Language: English
• Subjects: Animals; Biological and medical sciences; Blood Glucose - analysis; euglycaemic hyperinsulinaemic clamp; Fundamental and applied biological sciences. Psychology; Glucose - administration & dosage; Glucose Clamp Technique - methods; hyperglycaemia; hypoglycaemia; Infusions, Parenteral; Insulin - administration & dosage; Insulin - blood; Islet blood flow; Islets of Langerhans - blood supply; Male; MEDICIN; MEDICINE; Rats; Rats, Inbred WF; Regional Blood Flow - physiology; Vertebrates: anatomy and physiology, studies on body, several organs or systems
• ISSN: 1748-1708; 1748-1716; 1748-1716
• DOI: 10.1111/j.1748-1716.2006.01666.x

Aims:  Previous studies have demonstrated that pancreatic islet blood flow is crucially dependent on blood glucose concentration. Thus, hyperglycaemia increases and hypoglycaemia decreases islet blood perfusion, by a combination of nervous and metabolic signals. The aim of the present study was to evaluate if hyperinsulinaemia, without associated hypoglycaemia, affects islet blood flow. Methods:  Thiobutabarbital‐anaesthetized Wistar–Furth rats were subjected to an euglycaemic, hyperinsulinaemic clamp, that is they were infused for 60 min with either saline, insulin (18 mU kg−1 min−1), glucose (27 mg kg−1 min−1) or both glucose and insulin. This was followed by islet blood flow measurements with a microsphere technique. Results:  Animals receiving only glucose doubled their blood glucose and serum insulin concentrations, whereas rats receiving only insulin had blood glucose concentrations