Association of cystatin C with prostasomes in human seminal plasma

Summary It was recently elucidated that cystatin C, a protein targeted to the classical secretory pathway by its signal peptide sequence, can also be secreted in association with exosomes. Accordingly, we wanted to investigate whether there is a secretory link between cystatin C and prostasomes in h...

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Bibliographic Details
Published in:International journal of andrology 2011-08, Vol.34 (4pt1), p.363-368
Main Authors: Carlsson, L., Ronquist, G., Eliasson, R., Egberg, N., Larsson, A.
Format: Article
Language:English
Subjects:
Biological and medical sciences
Blotting, Western
cystatin C
Cystatin C - metabolism
Flow Cytometry
Fundamental and applied biological sciences. Psychology
Gynecology. Andrology. Obstetrics
Humans
Male
Male genital diseases
Mammalian male genital system
Medical sciences
MEDICIN
MEDICINE
prostasomes
Prostate - cytology
Prostate - metabolism
Semen - metabolism
seminal plasma
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
turbidimetric assay
Vertebrates: reproduction
Citations: Items that cite this one
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Summary:Summary It was recently elucidated that cystatin C, a protein targeted to the classical secretory pathway by its signal peptide sequence, can also be secreted in association with exosomes. Accordingly, we wanted to investigate whether there is a secretory link between cystatin C and prostasomes in human seminal plasma. Cystatin C concentrations in seminal plasma from 50 men including 6 vasectomized men were measured by turbidimetry on an Architect Ci8200. Some of the seminal plasma samples were also analysed utilizing an Epics Profile XL‐MCL cytometer. We found high concentrations of cystatin C in seminal plasma. The 2.5–97.5 percentiles, performed by bootstrap estimation, were 25.8 [95% confidence interval (CI): 22.3–29.4] to 77.0 mg/L (95% CI: 71.9–82.1). Cystatin C is present in approximately 50 times higher concentration in seminal plasma compared with blood plasma. There was no clear difference as regards seminal plasma content of cystatin C between vasectomized men and the rest of the group. Immunoblot analysis with chicken anti‐cystatin C antibody revealed a firm association of cystatin C with prostasomes. Flow cytometric analysis demonstrated that cystatin C was linked to prostasomes also meaning an at least partial prostasomal membrane surface localization.
ISSN:0105-6263
1365-2605
1365-2605
DOI:10.1111/j.1365-2605.2010.01090.x