Sensitive Detection of Spores Using Volume-Amplified Magnetic Nanobeads

A magnetic‐nanobead‐based, substrate‐free method for the sensitive detection of spores in an immunoassay format is presented. The method is shown to detect Bacillus globigii spores, the non‐pathogenic simulant of Bacillus anthracis, with a limit‐of‐detection of 50 spores with a reaction time of 135...

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Bibliographic Details
Published in:Small (Weinheim an der Bergstrasse, Germany) Germany), 2012-07, Vol.8 (14), p.2174-2177
Main Authors: Gómez de la Torre, Teresa Zardán, Ke, Rongqin, Mezger, Anja, Svedlindh, Peter, Strømme, Maria, Nilsson, Mats
Format: Article
Language:English
Subjects:
Bacillus - chemistry
Biosensing Techniques - methods
biosensors
DNA - chemistry
Engineering Science with specialization in Nanotechnology and Functional Materials
Engineering Science with specialization in Solid State Physics
Immunoassay - methods
immunoassays
magnetic particles
Magnetics
Magnetite Nanoparticles - chemistry
nanobeads
Sensitivity and Specificity
spores
Spores, Bacterial - chemistry
Spores, Bacterial - isolation & purification
TECHNOLOGY
TEKNIKVETENSKAP
Teknisk fysik med inriktning mot fasta tillståndets fysik
Teknisk fysik med inriktning mot nanoteknologi och funktionella material
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Summary:A magnetic‐nanobead‐based, substrate‐free method for the sensitive detection of spores in an immunoassay format is presented. The method is shown to detect Bacillus globigii spores, the non‐pathogenic simulant of Bacillus anthracis, with a limit‐of‐detection of 50 spores with a reaction time of 135 min. The study shows the versatility of magnetic nanobeads for detection of biological molecules other than DNA.
ISSN:1613-6810
1613-6829
DOI:10.1002/smll.201102632