Locally increased concentrations of inflammatory cytokines in an experimental intraabdominal adhesion model

• Published in: Journal of pediatric surgery 2014-10, Vol.49 (10), p.1480-1484
• Main Authors: Fredriksson, F, Christofferson, R.H, Carlsson, P.O, Lilja, H.E
• Format: Article
• Language: English
• Subjects: Animals; Ascitic Fluid - chemistry; Cytokines - analysis; Cytokines - blood; Disease Models, Animal; Immunoenzyme Techniques; Inflammatory cytokines; Intercellular Signaling Peptides and Proteins - analysis; Intercellular Signaling Peptides and Proteins - blood; Luminescent Measurements; Male; Pediatrics; Peritoneal adhesions; Peritoneal Diseases - blood; Rat; Rats; Rats, Sprague-Dawley; Surgery; Tissue Adhesions - blood; Tissue Adhesions - etiology
• ISSN: 0022-3468; 1531-5037; 1531-5037
• DOI: 10.1016/j.jpedsurg.2014.03.010

Abstract Background Peritoneal adhesions may cause bowel obstruction, infertility, and pain. This study investigated cytokines, proteins and growth factors thought to promote formation of adhesions in an experimental intraabdominal adhesion model. Methods Male Sprague-Dawley rats were subjected to laparotomy, cecal abrasion, and construction of a small bowel anastomosis and examined at various time points after surgery. Concentrations of cytokines and growth factors in plasma and peritoneal fluid were analyzed using electrochemoluminescence and quantitative sandwich enzyme immunoassay technique. Results Concentrations of interleukin-6 (IL-6), interleukin-1beta (IL-1β), and tumor necrosis factor alpha (TNF-α) increased in peritoneal fluid from 6 h after incision. Plasma concentrations of IL-6 increased at 6 h, but plasma concentrations of IL-1β and TNF-α remained low. Peritoneal fluid concentrations of platelet-derived growth factor-BB (PDGF-BB), transforming growth factor beta1 (TGF-β1), vascular endothelial growth factor (VEGF), tissue-type plasminogen activator (tPA) and plasminogen activator inhibitor-1 (PAI-1) were below detection levels at all time points. Conclusion Early elevations of IL-6, IL-1β, and TNF-α concentrations in peritoneal fluid correlated to adhesion formation in this rodent model. Our model is relevant and reproducible, suitable for intervention, and indicates that antiadhesion strategies should be early, local and not systemic.