Seeds to crystals

Seeding has been critical for obtaining diffraction-quality crystals for many structures. In this article, applications and recommendations for seeding are presented based on examples from our laboratory and other groups. The implementation of seeding in high-throughput crystallization, robotics, an...

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Bibliographic Details
Published in:Journal of structural biology 2003-04, Vol.142 (1), p.66-76
Main Author: Bergfors, Terese
Format: Article
Language:English
Subjects:
Biopolymers - chemistry
Cold Temperature
Crystallization
Crystallization - methods
Helium
Macromolecular Substances
Macroseeding
Microseeding
Nucleation
optimization
Ostwald ripening
protein crystallization
Seeding
Streak-seeding
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Description
Summary:Seeding has been critical for obtaining diffraction-quality crystals for many structures. In this article, applications and recommendations for seeding are presented based on examples from our laboratory and other groups. The implementation of seeding in high-throughput crystallization, robotics, and other emerging technologies is also discussed.
ISSN:1047-8477
1095-8657
DOI:10.1016/S1047-8477(03)00039-X