Receptor-mediated Mn2+ influx in rat hepatocytes : comparison of cells loaded with Fura-2 ester and cells microinjected with Fura-2 salt

In single Fura-2 ester-loaded hepatocytes, stimulation by vasopressin, but not emptying of the agonist-sensitive Ca2+ store by 2,5-di-(t-butyl)hydroquinone, resulted in an increase in the rate of Fura-2 fluorescence-quenching by Mn2+. Similarly, in cells microinjected with Fura-2 salt, vasopressin s...

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Bibliographic Details
Published in:Biochemical journal 1994-08, Vol.302 (1), p.5-9
Main Authors: KASS, G. E. N, WEBB, D.-L, CHOW, S. C, LLOPIS, J, BERGGREN, P.-O
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Biological Transport
Calcium - metabolism
Cell physiology
Esters
Fundamental and applied biological sciences. Psychology
Fura-2
Hydroquinones - pharmacology
Inositol 1,4,5-Trisphosphate - metabolism
Liver - drug effects
Liver - metabolism
Male
Manganese - metabolism
Microinjections
Molecular and cellular biology
Rats
Rats, Wistar
Receptors, Cell Surface - metabolism
Salts
Signal Transduction
Spectrometry, Fluorescence
Terpenes - pharmacology
Thapsigargin
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Summary:In single Fura-2 ester-loaded hepatocytes, stimulation by vasopressin, but not emptying of the agonist-sensitive Ca2+ store by 2,5-di-(t-butyl)hydroquinone, resulted in an increase in the rate of Fura-2 fluorescence-quenching by Mn2+. Similarly, in cells microinjected with Fura-2 salt, vasopressin stimulated Mn2+ entry while 2,5-di-(t-butyl)hydroquinone or thapsigargin did not. The pattern of Fura-2 quenching by Mn2+ only correlated with the movement of Mn2+ across the plasma membrane.
ISSN:0264-6021
1470-8728
1470-8728
DOI:10.1042/bj3020005