Choice of molecular barcode will affect species prevalence but not bacterial community composition

The rapid advancement of next generation sequencing protocols in recent years has led to the diversification in the methods used to study microbial communities; however, how comparable the data generated from these different methods are, remains unclear. In this study we compared the taxonomic compo...

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Bibliographic Details
Published in:Marine genomics 2016-10, Vol.29, p.39-43
Main Authors: Lebret, Karen, Schroeder, Joanna, Balestreri, Cecilia, Highfield, Andrea, Cummings, Denise, Smyth, Tim, Schroeder, Declan
Format: Article
Language:English
Subjects:
16S
Bacteria - classification
Bacteria - genetics
Biodiversity
Genomics - methods
Illumina
Marine bacterial community
Metagenome
Method Paper
Pyrosequencing
RNA, Ribosomal, 16S - genetics
Seasons
Sequence Analysis, RNA
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Summary:The rapid advancement of next generation sequencing protocols in recent years has led to the diversification in the methods used to study microbial communities; however, how comparable the data generated from these different methods are, remains unclear. In this study we compared the taxonomic composition and seasonal dynamics of the bacterial community determined by two distinct 16s amplicon sequencing protocols: sequencing of the V6 region of the 16s rRNA gene using 454 pyrosequencing vs the V4 region of the 16s rRNA gene using the Illumina Hiseq 2500 platform. Significant differences between relative abundances at all taxonomic levels were observed; however, their seasonal dynamics between phyla were largely consistent between methods. This study highlights that care must be taken when comparing datasets generated from different methods.
ISSN:1874-7787
1876-7478
1876-7478
DOI:10.1016/j.margen.2016.09.001