Enhancement of Cell Adhesion on a Phosphorylcholine-Based Surface through the Interaction with DNA Mediated by Ca2+ Ions

2-Methacryloyloxyethyl phosphorylcholine (MPC) has a PC group and is one of the most well-known bioinert polymers. In this study, we evaluated the interaction between MPC and DNA, which specifically interacts with the phospholipid head group via Ca2+ ions. A MPC monolayer and poly­(MPC) brush were f...

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Bibliographic Details
Published in:The journal of physical chemistry. B 2016-12, Vol.120 (48), p.12272-12278
Main Authors: Azuma, Tomoyuki, Teramura, Yuji, Hoshi, Toru, Takai, Madoka
Format: Article
Language:English
Online Access:Get full text
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Summary:2-Methacryloyloxyethyl phosphorylcholine (MPC) has a PC group and is one of the most well-known bioinert polymers. In this study, we evaluated the interaction between MPC and DNA, which specifically interacts with the phospholipid head group via Ca2+ ions. A MPC monolayer and poly­(MPC) brush were fabricated to observe the effect of the structure on the interaction between MPC and DNA via Ca2+ ions. The poly­(MPC) brush, which shows higher MPC unit density, more efficiently interacted with DNA via Ca2+ ions. Also, serum protein could interact with the poly­(MPC) brush via DNA, although the brush itself hardly interacted with serum proteins. Cell adhesion was significantly provoked on poly­(MPC)/DNA compared with poly­(MPC) because serum protein adsorption was induced on poly­(MPC)/DNA.
ISSN:1520-6106
1520-5207
1520-5207
DOI:10.1021/acs.jpcb.6b08741