Improved method for quantitative analysis of the cyclotide kalata B1 in plasma and brain homogenate

This study provides a new method for quantifying the cyclotide kalata B1 in both plasma and brain homogenate. Cyclotides are ultra‐stable peptides with three disulfide bonds that are interesting from a drug development perspective as they can be used as scaffolds. In this study we describe a new val...

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Bibliographic Details
Published in:Biopolymers 2016-11, Vol.106 (6), p.910-916
Main Authors: Melander, Erik, Eriksson, Camilla, Jansson, Britt, Göransson, Ulf, Hammarlund-Udenaes, Margareta
Format: Article
Language:English
Subjects:
Animals
Binding
Brain
Brain - metabolism
Chromatography
cyclotides
Cyclotides - pharmacokinetics
Cyclotides - pharmacology
In vivo methods and tests
kalata B1
liquid chromatography
Liquids
Male
Mass Spectrometry
Models, Biological
Particle size
pharmacokinetics
Pharmacology
Plasma - metabolism
Quantitative analysis
Rats
Rats, Sprague-Dawley
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Summary:This study provides a new method for quantifying the cyclotide kalata B1 in both plasma and brain homogenate. Cyclotides are ultra‐stable peptides with three disulfide bonds that are interesting from a drug development perspective as they can be used as scaffolds. In this study we describe a new validated LC‐MS/MS method with high sensitivity and specificity for kalata B1. The limit of quantification was 2 ng/mL in plasma and 5 ng/gmL in brain homogenate. The method was linear in the range 2–10,000 ng/mL for plasma and 5–2000 ng/g for brain. Liquid Chromatographic separation was performed on a HyPurity C18 column, 50 × 4.6 mm, 3 µm particle size. The method had inter‐ and intra‐day precision and accuracy levels
ISSN:0006-3525
1097-0282
1097-0282
DOI:10.1002/bip.22984