Role of BDNF and neurotrophic receptors in human inner ear development

• Published in: Cell and tissue research 2017-12, Vol.370 (3), p.347-363
• Main Authors: Johnson Chacko, L., Blumer, M. J. F., Pechriggl, E., Rask-Andersen, H., Dietl, W., Haim, A., Fritsch, H., Glueckert, R., Dudas, J., Schrott-Fischer, A.
• Format: Article
• Language: English
• Subjects: Animals; Base Sequence; BDNF; Biomedical and Life Sciences; Biomedicine; Brain-derived neurotrophic factor; Brain-Derived Neurotrophic Factor - metabolism; Cochlea; Cochlea - embryology; Cochlea - metabolism; Embryogenesis; Facial nerve; Fetuses; Fibers; Gene expression; Gene Expression Regulation, Developmental; Gestational age; Hair; Hair cells; Hearing protection; Human; Human Genetics; Humans; Immunohistochemistry; In situ hybridization; Inner ear; Localization; Membrane Glycoproteins - metabolism; Mice; Molecular Medicine; Nerve Growth Factors - metabolism; Nerve Tissue Proteins - metabolism; Neurons; Neurotrophin receptors; Proteomics; Receptor, trkA - metabolism; Receptor, trkB - metabolism; Receptor, trkC - metabolism; Receptors, Nerve Growth Factor - metabolism; Regular Article; RNA; Saccule; Sensory neurons; Subpopulations; Trk receptors; TrkA protein; TrkA receptors; TrkB receptors; Utricle; Vestibular system
• ISSN: 0302-766X; 1432-0878; 1432-0878
• DOI: 10.1007/s00441-017-2686-9

The expression patterns of the neurotrophin, brain-derived neurotrophic factor, BDNF, and the neurotrophic receptors—p75NTR and Trk receptors—in the developing human fetal inner ear between the gestational weeks (GW) 9 to 12 are examined via in situ hybridization and immunohistochemistry. BDNF mRNA expression was highest in the cochlea at GW 9 but declined in the course of development. In contrast to embryonic murine specimens, a decline in BDNF expression from the apical to the basal turn of the cochlea could not be observed. p75NTR immunostaining was most prominent in the nerve fibers that penetrate into the sensory epithelia of the cochlea, the urticule and the saccule as gestational age progresses. TrkB and TrkC expression intensified towards GW 12, at which point the BDNF mRNA localization was at its lowest. TrkA expression was limited to fiber subpopulations of the facial nerve at GW 10. In the adult human inner ear, we observed BDNF mRNA expression in the apical poles of the cochlear hair cells and supporting cells, while in the adult human utricle, the expression was localized in the vestibular hair cells. We demonstrate the highly specific staining patterns of BDNF mRNA and its putative receptors over a developmental period in which multiple hearing disorders are manifested. Our findings suggest that BDNF and neurotrophin receptors are important players during early human inner ear development. In particular, they seem to be important for the survival of the afferent sensory neurons.