Protective Effect of Tamarind Seed Coat Ethanol Extract on Eryptosis Induced by Oxidative Stress

• Published in: Biochemistry (Moscow) 2020, Vol.85 (1), p.119-129
• Main Authors: Kengaiah, J., Nandish, S. K. M., Ramachandraiah, C., Chandramma, Shivaiah, A., Vishalakshi, G. J., Paul, M., Santhosh, M. S., Shankar, R. L., Sannaningaiah, Devaraja
• Format: Article
• Language: English
• Subjects: 2 '-azobis(2-amidinopropane) dihydrochloride; Acceleration; Alcohol; Alcohol, Denatured; Analysis; Anemia; Antioxidants; Antioxidants - pharmacology; Apoptosis; Biochemistry; Biomarkers - metabolism; Biomedical and Life Sciences; Biomedicine; Bioorganic Chemistry; Calcium (intracellular); Calcium - metabolism; Carbonyl compounds; Carbonyls; Causes of; Cell death; Coating effects; Diabetes mellitus; Enzymes; eryptosis; Eryptosis - drug effects; Erythrocytes; Erythrocytes - cytology; Erythrocytes - drug effects; Ethanol; Fourier transforms; glucose-6-phosphate dehydrogenase; Glutathione; Glutathione - metabolism; High-performance liquid chromatography; Humans; Infrared spectroscopy; Kidney diseases; Life Sciences; Lipid Peroxidation; Lipids; Liquid chromatography; Metabolites; Microbiology; Oxidation; Oxidative stress; Oxidative Stress - drug effects; Peroxidation; Phosphatidylserine; Plant Extracts - pharmacology; Production processes; Reactive oxygen species; Reactive Oxygen Species - metabolism; Replenishment; Secondary metabolites; Seed coats; Seeds - metabolism; Sepsis; Tamarind; tamarind seed coat ethanol extract; Tamarindus - metabolism
• ISSN: 0006-2979; 1608-3040; 1608-3040
• DOI: 10.1134/S0006297920010113

Suicidal erythrocyte death, or eryptosis, is the key event in eliciting anemia in numerous pathological conditions, including diabetes, chronic kidney disease, cancer, sepsis, etc. Oxidative stress is an important trigger in the acceleration of erythrocyte loss via eryptosis and an underlying mechanism of anemia emergence in the above pathologies. Therefore, there is an increasing demand for identification of antioxidants and anti-eryptotic agents forthe management of stress-related ailments. Here, we demonstrated the antioxidant and anti-eryptotic properties of the tamarind seed coat ethanol extract (TSCEE) against 2,2'-azobis(2-amidinopropane) dihydrochloride (AAPH)-induced oxidative stress and eryptosis. The presence of probable secondary metabolites in the TSCEE extract was investigated by RP-HPLC. Active groups present in the TSCEE were studied by the Fourier-transform infrared spectroscopy. Cyclic voltammetric studies confirmed the antioxidant potential of TSCEE. The protective effect of TSCEE on red blood cells was confirmed by assessing various eryptotic markers, such as reactive oxygen species generation, intracellular calcium levels, and phosphatidylserine exposure. TSCEE reduced lipid peroxidation and protein carbonyl content and restored the levels of glutathione, antioxidant enzymes, and enzymes involved in glutathione replenishment. In conclusion, TSCEE was found to exhibit multiple therapeutic properties, which makes it a. promising agent for treating oxidative stress-induced eryptosis and subsequent anemia in various pathologies.