Selective in vivo molecular and cellular biocompatibility of black peppercorns by piperine-protein intrinsic atomic interaction with elicited oxidative stress and apoptosis in zebrafish eleuthero embryos

• Published in: Ecotoxicology and environmental safety 2020-04, Vol.192, p.110321, Article 110321
• Main Authors: Patel, Paritosh, Panda, Pritam Kumar, Kumari, Puja, Singh, Puneet Kumar, Nandi, Aditya, Mallick, M.A., Das, Biswadeep, Suar, Mrutyunjay, Verma, Suresh K.
• Format: Article
• Language: English
• Subjects: Alkaloids - analysis; Alkaloids - chemistry; Animals; Apoptosis; Apoptosis - drug effects; Benzodioxoles - analysis; Benzodioxoles - chemistry; Biocompatibility; Black pepper; Oxidative stress; Oxidative Stress - drug effects; Piper nigrum - chemistry; Piper nigrum - embryology; Piper nigrum - toxicity; Piperidines - analysis; Piperidines - chemistry; Piperine; Plant Extracts - chemistry; Plant Extracts - toxicity; Polyunsaturated Alkamides - analysis; Polyunsaturated Alkamides - chemistry; Seeds - chemistry; Seeds - toxicity; Superoxide Dismutase - metabolism; Tumor Suppressor Protein p53 - metabolism; Zebrafish; Zebrafish - embryology; Zebrafish - physiology; Zebrafish Proteins - chemistry; Zebrafish Proteins - metabolism
• ISSN: 0147-6513; 1090-2414; 1090-2414
• DOI: 10.1016/j.ecoenv.2020.110321

Day to day consumption of black pepper raise concern about the detailed information about their medicinal, pharmaceutical values and knowledge about the biocompatibility with respect to ecosystem. This study investigates the in vivo selective molecular biocompatibility of its seed cover (SC) and seed core (SP) powder extract using embryonic zebrafish model. Gas chromatography mass spectrometry (GCMS) analysis of the extract prepared by grinding showed presence of different components with “piperine” as principle component. Biocompatibility analysis showed dose and time dependent selective effect of SC and SP with LC50 of 30.4 μg/ml and 35.6 μg/ml, respectively on survivability, hatching and heartbeat rate in embryonic zebrafish. Mechanistic investigation elucidated it as effect of accumulation and internalization of black pepper leading to their influence on structure and function of cellular proteins hatching enzyme (he1a), superoxide dismutase (sod1) and tumor protein (tp53) responsible for delayed hatching, oxidative stress induction and apoptosis. The study provided insight to selective biocompatibility of black pepper expedient to produce higher quality spices with respect to pharmaceutical, clinical and environmental aspects. [Display omitted] 1.Seed cover (SC) and seed core (SP) part of black peppercorn seeds were tested for their differential toxicity.2.Selective biocompatibility analysis showed differential effect of Seed cover (SC) and seed core (SP) on zebrafish metabolic activities.3.Pepper showed molecular interaction with he1a, Sod1 and tp53 enzymes to influence biocompatibility.