Propionibacterium acnes and lipopolysaccharide induce the expression of antimicrobial peptides and proinflammatory cytokines/chemokines in human sebocytes

• Published in: Microbes and infection 2006-07, Vol.8 (8), p.2195-2205
• Main Authors: Nagy, István, Pivarcsi, Andor, Kis, Kornélia, Koreck, Andrea, Bodai, László, McDowell, Andrew, Seltmann, Holger, Patrick, Sheila, Zouboulis, Christos C., Kemény, Lajos
• Format: Article
• Language: English
• Subjects: Acne; Antimicrobial Cationic Peptides - biosynthesis; Biological and medical sciences; Cell Line; Cell Survival; Coculture Techniques; Cytokines - biosynthesis; DNA, Bacterial - chemistry; DNA, Bacterial - genetics; Escherichia; Escherichia coli; Fundamental and applied biological sciences. Psychology; Gene Expression; Humans; Inflammation; Innate immunity; Lipopolysaccharides - immunology; Microbiology; Molecular Sequence Data; Phylogeny; Pilosebaceous unit; Propionibacterium acnes; Propionibacterium acnes - immunology; Propionibacterium acnes - isolation & purification; Rec A Recombinases - genetics; RNA - analysis; RNA - genetics; Sebaceous Glands - cytology; Sebaceous Glands - microbiology; Sebocytes; Sequence Analysis, DNA
• ISSN: 1286-4579; 1769-714X; 1769-714X
• DOI: 10.1016/j.micinf.2006.04.001

Acne is a common skin disorder of the pilosebaceous unit. In addition to genetic, hormonal and environmental factors, abnormal colonization by Propionibacterium acnes has been implicated in the occurrence of acne via the induction of inflammatory mediators. To gain more insight into the role that sebocytes play in the innate immune response of the skin, particularly in acne, we compared the antimicrobial peptide and proinflammatory cytokine/chemokine expression at mRNA and protein levels, as well as the viability and differentiation of SZ95 sebocytes in response to co-culture with representative isolates of P. acnes type IA and type IB as well as Escherichia coli-derived lipopolysaccharide (LPS). We found that, in vitro, P. acnes type IA and IB isolates and LPS induced human β-defensin-2 and proinflammatory cytokine/chemokine expression, and influenced sebocyte viability and differentiation. Our results provide evidence that sebocytes are capable of producing proinflammatory cytokines/chemokines and antimicrobial peptides, which may have a role in acne pathogenesis. Furthermore, since P. acnes types IA and IB differentially affect both the differentiation and viability of sebocytes, our data demonstrate that different strains of P. acnes vary in their capacity to stimulate an inflammatory response within the pilosebaceous follicle.