Differentiation-regulated expression of Toll-like receptors 2 and 4 in HaCaT keratinocytes

• Published in: Archives of Dermatological Research 2004-08, Vol.296 (3), p.120-124
• Main Authors: PIVARCSI, Andor, KORECK, Andrea, BODAI, Laszlo, SZELL, Marta, SZEG, Csilla, BELSO, Nora, KENDERESSY-SZABO, Anna, BATA-CSÖRG, Zsuzsanna, DOBOZY, Attila, KEMENY, Lajos
• Format: Article
• Language: English
• Subjects: Biological and medical sciences; Cell Differentiation - physiology; Cell Line; Dermatology; Epidermis - cytology; Epidermis - immunology; Gene Expression - immunology; Humans; Keratinocytes - cytology; Keratinocytes - physiology; Medical sciences; Membrane Glycoproteins - genetics; Receptors, Cell Surface - genetics; RNA, Messenger - analysis; Toll-Like Receptor 2; Toll-Like Receptor 4; Toll-Like Receptors; Up-Regulation - immunology
• ISSN: 0340-3696; 1432-069X; 1432-069X
• DOI: 10.1007/s00403-004-0475-2

Toll-like receptors (TLRs) play an important role in the recognition of pathogens in keratinocytes. In this study, we investigated whether the differentiation state of HaCaT keratinocytes correlates with the expression of TLR2 and TLR4 genes. The expression levels of TLR2 and TLR4 in a HaCaT differentiation model system were determined using quantitative real-time RT-PCR (Q-RT-PCR) and flow cytometry. The progression of keratinocyte differentiation was monitored by determining the level of involucrin gene expression using Q-RT-PCR. The expression levels of TLR2 and TLR4 increased with the stage of differentiation and there were strong correlations between the expression level of the involucrin gene and those of the TLR2 gene ( r=0.809, P