Biocompatible biogenic silver nanoparticles interact with caspases on an atomic level to elicit apoptosis

To synthesize biocompatible and ecofriendly silver nanoparticles (AgNPs) and elucidate their molecular and cellular nanotoxicity at an atomic level. AgNPs were biosynthesized using Andrographolide and their cellular and molecular biocompatibility was evaluated using zebrafish embryos. AgNPs with a s...

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Bibliographic Details
Published in:Nanomedicine (London, England) England), 2020-09, Vol.15 (22), p.2119-2132
Main Authors: Kumari, Shalini, Kumari, Puja, Panda, Pritam K, Patel, Paritosh, Jha, Ealisha, Mallick, M Anwar, &, Mrutyunjay Suar, Verma, Suresh K
Format: Article
Language:English
Subjects:
Andrographis paniculata
Apoptosis
Biocompatibility
biological synthesis
caspase
Cytotoxicity
Eggs
Metabolism
Nanomaterials
Nanoparticles
Reactive oxygen species
ROS
Silver
silver nanoparticles
Spectrum analysis
Zebrafish
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Summary:To synthesize biocompatible and ecofriendly silver nanoparticles (AgNPs) and elucidate their molecular and cellular nanotoxicity at an atomic level. AgNPs were biosynthesized using Andrographolide and their cellular and molecular biocompatibility was evaluated using zebrafish embryos. AgNPs with a size of 80 nm and ζ potential of -52 mV were obtained. The LC50 for the AgNPs embryonic zebrafish was found to be 125 μg/ml. AgNPs induced reactive oxygen species production and elicit apoptosis mechanistically, then nanoparticles were shown to interact with caspase-3 and caspase-9 proteins through proline, cysteine, glycine and histidine amino acid residues via H-bond of corresponding bond energies. Biosynthesized AgNPs have potential to be used for biomedical and therapeutic applications.
ISSN:1743-5889
1748-6963
1748-6963
DOI:10.2217/nnm-2020-0138