Comparison between Abbott m2000 RealTime and Alinity m STI systems for detection of Chlamydia trachomatis, Neisseria gonorrhoeae, and Mycoplasma genitalium

• Published in: European journal of clinical microbiology & infectious diseases 2021-10, Vol.40 (10), p.2217-2220
• Main Authors: Herrmann, Björn, Malm, Karin
• Format: Article
• Language: English
• Subjects: Assaying; Automation - instrumentation; Automation - methods; Biomedical and Life Sciences; Biomedicine; Brief Report; Chlamydia; Chlamydia Infections - diagnosis; Chlamydia Infections - microbiology; Chlamydia trachomatis; Chlamydia trachomatis - genetics; Chlamydia trachomatis - isolation & purification; Condoms; DNA, Bacterial - genetics; DNA, Bacterial - isolation & purification; Gonorrhea - diagnosis; Gonorrhea - microbiology; Humans; Internal Medicine; Medical Microbiology; Mycoplasma genitalium; Mycoplasma genitalium - genetics; Mycoplasma genitalium - isolation & purification; Mycoplasma Infections - diagnosis; Mycoplasma Infections - microbiology; Neisseria gonorrhoeae; Neisseria gonorrhoeae - genetics; Neisseria gonorrhoeae - isolation & purification; Nucleic acid amplification tests; Polymerase chain reaction; Real time; Real-Time Polymerase Chain Reaction - instrumentation; Real-Time Polymerase Chain Reaction - methods
• ISSN: 0934-9723; 1435-4373; 1435-4373
• DOI: 10.1007/s10096-020-04135-9

The new Abbott Alinity m STI Assay was compared with Abbott m2000 RealTime PCR. For Chlamydia trachomatis , 26 (7.5%) of 347 samples were positive in the Alinity assay and 24 (6.9%) in the m2000 assay. Corresponding figures for Neisseria gonorrhoeae were 23 (6.6%) and 17 (4.9%). For Mycoplasma genitalium , 22 (7.9%) of 279 samples were positive in the Alinity assay and 18 (6.5%) in the m2000 assay, for which DNA extraction was performed on an m2000sp instrument combined with in-house real-time PCR. The Alinity assay has at least the same sensitivity as the m2000 assay. The specificity was evaluated by discrepancy analysis.