A full-length infectious cDNA clone of a dsRNA totivirus-like virus

Totivirus-like viruses are a group of non-segmented double-stranded (ds)RNA viruses with two open reading frames, which were recently discovered and provisionally assigned to the Totiviridae family. Unlike yeast and protozoan Totiviridae viruses, these totivirus-like viruses infect a diverse spectru...

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Bibliographic Details
Published in:Virology (New York, N.Y.) N.Y.), 2022-11, Vol.576, p.127-133
Main Authors: Wang, Han, de Matos Filipe, Diogo, Okamoto, Kenta
Format: Article
Language:English
Subjects:
dsRNA virus
Infectious clone
Mosquito
OmRV
Reverse genetics
Totivirus
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Summary:Totivirus-like viruses are a group of non-segmented double-stranded (ds)RNA viruses with two open reading frames, which were recently discovered and provisionally assigned to the Totiviridae family. Unlike yeast and protozoan Totiviridae viruses, these totivirus-like viruses infect a diverse spectrum of metazoan hosts and currently have enormous impacts on fisheries and agriculture. We developed the first infectious full-length cDNA clone of a totivirus-like virus, the Omono River virus (OmRV), and produced infectious particles using an RNA-transcript-based method. Compared with the parent wild-type particles from nature, the infectious-cloning OmRV particles have presented strong cytopathic effects, infectivity and similar morphology. Thus far, the established system is one of the few reported systems for generating a non-segmented dsRNA virus cDNA clone. •The first synthetic transcript of a metazoan dsRNA totivirus-like virus.•The synthetic particles were intensively assessed on their morphology and infectivity.•One function-deficient mutant T365A reveals the importance of the protrusion protein.
ISSN:0042-6822
1096-0341
1096-0341
DOI:10.1016/j.virol.2022.09.010