Expression of intestinal drug transporter proteins and metabolic enzymes in neonatal and pediatric patients

• Published in: International journal of pharmaceutics 2024-04, Vol.654, p.123962-123962, Article 123962
• Main Authors: de Waal, Tom, Handin, Niklas, Brouwers, Joachim, Miserez, Marc, Hoffman, Ilse, Rayyan, Maissa, Artursson, Per, Augustijns, Patrick
• Format: Article
• Language: English
• Subjects: Adult; Carrier Proteins; Child; Drug transporter proteins; Gastrointestinal ontogeny; Humans; Imidazoles; Infant, Newborn; Intestine, Small - metabolism; Liver - metabolism; Membrane Transport Proteins - metabolism; Metabolic enzymes; Neonates; Organosilicon Compounds; Pediatric patients
• ISSN: 0378-5173; 1873-3476; 1873-3476
• DOI: 10.1016/j.ijpharm.2024.123962

[Display omitted] The development of pediatric oral drugs is hampered by a lack of predictive simulation tools. These tools, in turn, require data on the physiological variables that influence oral drug absorption, including the expression of drug transporter proteins (DTPs) and drug-metabolizing enzymes (DMEs) in the intestinal tract. The expression of hepatic DTPs and DMEs shows age-related changes, but there are few data on protein levels in the intestine of children. In this study, tissue was collected from different regions of the small and large intestine from neonates (i.e., surgically removed tissue) and from pediatric patients (i.e., gastroscopic duodenal biopsies). The protein expression of clinically relevant DTPs and DMEs was determined using a targeted mass spectrometry approach. The regional distribution of DTPs and DMEs was similar to adults. Most DTPs, with the exception of MRP3, MCT1, and OCT3, and all DMEs showed the highest protein expression in the proximal small intestine. Several proteins (i.e., P-gp, ASBT, CYP3A4, CYP3A5, CYP2C9, CYP2C19, and UGT1A1) showed an increase with age. Such increase appeared to be even more pronounced for DMEs. This exploratory study highlights the developmental changes in DTPs and DMEs in the intestinal tract of the pediatric population. Additional evaluation of protein function in this population would elucidate the implications of the presented changes in protein expression on absorption of orally administered drugs in neonates and pediatric patients.