Proximal discrepancies in intrinsic atomic interaction determines comparative in vivo biotoxicity of Chlorpyrifos and 3,5,6-trichloro-2-pyridinol in embryonic zebrafish

• Published in: The Science of the total environment 2024-02, Vol.913, p.169780-169780, Article 169780
• Main Authors: Ghosh, Aishee, Singh, Sonal, Saha, Utsa, Jena, Snehasmita, Simnani, Faizan Zareen, Singh, Dibyangshee, Gupta, Abha, Nandi, Aditya, Sinha, Adrija, Nayak, Tanmaya, Rout, Prabhat Kumar, Panda, Pritam Kumar, Singh, Deobrat, Raina, Vishakha, Verma, Suresh K.
• Format: Article
• Language: English
• Subjects: 3,5,6-Trichloro-2-pyridinol; 6-Trichloro-2-pyridinol; Apoptosis; Biotoxicity; Chlorpyrifos; Oxidative stress; Zebrafish
• ISSN: 0048-9697; 1879-1026; 1879-1026
• DOI: 10.1016/j.scitotenv.2023.169780

Bioaccumulation of Chlorpyrifos (CP) as pesticides due to their aggrandized use in agriculture has raised serious concern on the health of ecosystem and human beings. Moreover, their degraded products like 3,5,6-trichloro-2-pyridinol (TCP) has enhanced the distress due to their unpredictable biotoxicity. This study evaluates and deduce the comparative in vivo mechanistic biotoxicity of CP and TCP with zebrafish embryos through experimental and computational approach. Experimental cellular and molecular analysis showed higher induction of morphological abnormalities, oxidative stress and apoptosis in TCP exposed embryos compared to CP exposure due to upregulation of metabolic enzymes like Zhe1a, Sod1 and p53. Computational analysis excavated the differential discrepancies in intrinsic atomic interaction as a reason of disparity in biotoxicity of CP and TCP. The mechanistic differences were deduced due to the differential accumulation and internalisation leading to variable interaction with metabolic enzymes for oxidative stress and apoptosis causing physiological and morphological abnormalities. The study unravelled the information of in vivo toxicity at cellular and molecular level to advocate the attention of taking measures for management of CP as well as TCP for environmental and human health. [Display omitted] •Chlorpyrifos (CP) and 3,5,6-trichloro-2-pyridinol (TCP) bioaccumulation possess in environmental concern of toxicity.•Uptake of TCP in zebrafish embryos is higher than CP due to molecular structure.•TCP induces higher deformities, oxidative stress and apoptosis than CP in zebrafish embryos.•Intrinsic atomic interaction of TCP with Sod1, Zhe1a and p53 proteins vary at molecular level.•Proximal discrepancies in intrinsic atomic interaction of CP and TCP determines in vivo biotoxicity in zebrafish embryos.