An i-type lysozyme from a crustacean, Pacifastacus leniusculus, functions as a clot-destabilising enzyme

• Published in: Fish & shellfish immunology 2024-09, Vol.152, p.109769, Article 109769
• Main Authors: Ekblom, Charlotta, Söderhäll, Kenneth, Söderhäll, Irene
• Format: Article
• Language: English
• Subjects: Amino Acid Sequence; Animals; Arthropod Proteins - chemistry; Arthropod Proteins - genetics; Arthropod Proteins - immunology; Astacoidea - genetics; Astacoidea - immunology; Base Sequence; Blood Coagulation - drug effects; Clotting protein; Crustacean; Destabilase; Gene Expression Profiling - veterinary; Hemocytes - immunology; I -type lysozyme; Immunity, Innate; Muramidase - chemistry; Muramidase - genetics; Muramidase - immunology; Muramidase - metabolism; Phylogeny; Sequence Alignment - veterinary; Transglutaminase
• ISSN: 1050-4648; 1095-9947; 1095-9947
• DOI: 10.1016/j.fsi.2024.109769

Lysozymes are hydrolytic enzymes, and they are ubiquitous among all living organisms. They are mostly associated with antibacterial properties through their muramidase activity, while other properties such as iso-peptidase activity are also common. Invertebrate-type (i-type) lysozymes include the enzyme Destabilase, which is present in the salivary secretions of the medicinal leach Hirundo medicinalis. Destabilase has the ability to hydrolyse the ε-(γ-glutamyl)-lysine iso-peptide bonds formed by transglutaminase in fibrin of vertebrate blood, thereby destabilising blood clots. We have identified an i-type lysozyme from the hemocytes of the freshwater crayfish Pacifastacus leniusculus, which was found to be upregulated at the protein level in response to an injection of the β-1,3-glucan laminarin. Based on its sequence we predicted that this lysozyme would lack muramidase activity, and therefore we decided to determine its putative immune function. The P. leniusculus i-type lysozyme (Pl-ilys), is a protein with 159 amino acid residues, including a 29 residue signal peptide, with a predicted molecular weight of 16 kDa and a predicted pI of 5.6. It is expressed primarily in the hemocytes and to a lesser extent in the hematopoietic tissue. A recombinant mature Pl-ilys using an E. coli expression system was produced, and we could ascertain that this enzyme was deficient of muramidase activity. Moreover, no iso-peptidase activity could be detected against the substrate l-γ-glutamine-p-nitroanilide. Analysis of the conserved domains in Pl-ilys showed a putative destabilase domain, and thus we tested the clot dissolving activity of this enzyme. We could show that the purified P. leniusculus clotting protein which had been coagulated and clotted with transglutaminase was dissolved by the addition of Pl-ilys. Taken together our results indicate that Pl-ilys has a clot dissolving or destabilising activity in crustacean blood. •Pacifastacus leniusculus i-type lysozyme (Pl-ilys) is mainly expressed in hemocytes.•Pl-ilys is deficient of muramidase activity.•Pl-ilys is functioning as a clot degrading or destabilising enzyme.