Aqueous two-phase systems as a formulation concept for spray-dried protein

This study investigates to what extent an aqueous two-phase system (ATPS) can encapsulate and protect the secondary structure of a protein during spray drying. The ATPSs contained polyvinyl alcohol (PVA) and dextran solutions, in different proportions. A model protein, bovine serum albumin (BSA) and...

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Bibliographic Details
Published in:International journal of pharmaceutics 2005-04, Vol.294 (1), p.73-87
Main Authors: Elversson, Jessica, Millqvist-Fureby, Anna
Format: Article
Language:English
Subjects:
Animals
Aqueous two-phase system
Biological and medical sciences
Bovine serum albumin
Cattle
Chemistry, Pharmaceutical
Electron spectroscopy for surface analysis
Encapsulation
Fourier transform infrared spectroscopy
General pharmacology
Medical sciences
Pharmaceutical technology. Pharmaceutical industry
Pharmacology. Drug treatments
Phase Transition
Protein formulation
Proteins - chemical synthesis
Serum Albumin, Bovine - chemical synthesis
Spray drying
Technology, Pharmaceutical - methods
Trehalose
Water - chemistry
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Summary:This study investigates to what extent an aqueous two-phase system (ATPS) can encapsulate and protect the secondary structure of a protein during spray drying. The ATPSs contained polyvinyl alcohol (PVA) and dextran solutions, in different proportions. A model protein, bovine serum albumin (BSA) and, in some experiments, trehalose were added to the ATPS prior to spray drying. Electron spectroscopy for chemical analysis (ESCA), differential scanning calorimetry (DSC), UV spectrophotometry, size exclusion high-performance liquid chromatography (SEC-HPLC) and Fourier transform infrared spectroscopy (FTIR) were used for analysis of solid and reconstituted samples. The anticipated function of the ATPS was to improve the stability of the protein by preventing interactions with the air–liquid interface during drying and by improving the encapsulation of the protein in the dried powder. BSA was found to preferentially partition to the dextran phase and in the absence of PVA, BSA dominated the powder surface. In samples containing PVA, the polymer mainly covered the powder surface, even though the dextran-rich phase was continuous, thus preventing protein surface interactions and providing improved encapsulation. However, PVA was found to cause partial loss of the native structure of BSA although the protein was well encapsulated during spray drying.
ISSN:0378-5173
1873-3476
1873-3476
DOI:10.1016/j.ijpharm.2005.01.015